Home » FGFR3 » FGFR3 Antibodies » FGFR3 Antibody (2H10B4)

FGFR3 Antibody (2H10B4)

 (1 Publication)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: FGF R3 Antibody (2H10B4) [NBP2-52468] - Analysis using FGFR3 mAb against human FGFR3 (AA: 529-694) recombinant protein. (Expected MW is 44.1 kDa)
Western Blot: FGF R3 Antibody (2H10B4) [NBP2-52468] - Analysis using FGFR3 mAb against human FGFR3 (AA: 529-694) recombinant protein. (Expected MW is 44.1 kDa)
Immunohistochemistry: FGF R3 Antibody (2H10B4) [NBP2-52468] - Analysis of paraffin-embedded ovarian cancer tissues using FGFR3 mouse mAb with DAB staining.
Flow Cytometry: FGF R3 Antibody (2H10B4) [NBP2-52468] - Analysis of A549 cells using FGFR3 mouse mAb (green) and negative control (red).
Western Blot: FGF R3 Antibody (2H10B4) [NBP2-52468] - Analysis using FGFR3 mAb against HEK293 (1) and FGFR3 (AA: 529-694)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: FGF R3 Antibody (2H10B4) [NBP2-52468] - Analysis of paraffin-embedded endometrial cancer tissues using FGFR3 mouse mAb with DAB staining.
ELISA: FGF R3 Antibody (2H10B4) [NBP2-52468] - Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
Clone
2H10B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

FGFR3 Antibody (2H10B4) Summary

Immunogen
Purified recombinant fragment of human FGFR3 (AA: 529-694) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FGFR3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • CyTOF-ready
Application Notes
This antibody is Cytof ready.
Theoretical MW
87.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
FGFR3 Knockout 293T Cell Lysate
Publications
Read Publication using
NBP2-52468 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for FGFR3 Antibody (2H10B4)

  • CD333
  • CEK
  • EC 2.7.10
  • FGF R3
  • FGFR3
  • fibroblast growth factor receptor 3
  • HSFGFR3EX
  • JTK4
  • JTK4thanatophoric dwarfism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

FGFR2 Antibody
(OTI5C5)
NBP2-00932
Western Blot: FGF R2 Antibody (5C5) [NBP2-00932] - Analysis of FGF R2 in HEK293T cells (left, control) and HEK293T cells overexpressing FGF R2 (right) using anti-FGF R2 antibody. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: FGFR2 Antibody (5C5) [NBP2-00932] Staining of COS7 cells transiently transfected by pCMV6-ENTRY FGFR2.
Immunohistochemistry-Paraffin: FGFR2 Antibody (5C5) [NBP2-00932] - Staining of paraffin-embedded Human endometrium tissue using anti-FGFR2 mouse monoclonal antibody.
(1 Review)
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
FGFR1 Antibody
(M19B2)
NB600-1287
Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.
Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis using the Alexa Fluor 488 (R) conjugate of NB600-1287. Staining of FGF R1 in HEK293 and HEK293 FGFR transfected cells using anti-FGF R1 antibody. (Red) Positive cells, (Green) Negative cells, (Blue) Negative cells + antibody, (Pink) Positive cells + antibody. Image from verified customer review.
Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using FGF R1 antibody (Dark Blue) and Isotype control (Orange). An antibody concentration of 1 ug/1x10^6 cells was used.
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IP, CyTOF-ready
FGF basic/FGF2
[Unconjugated]
233-FB
1 µg/lane of Recombinant Human FGF basic/FGF2 (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human FGF basic/FGF2 (146 aa) (Catalog # 233-FB) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.1-0.6 ng/mL.
(13 Reviews)
(314 Publications)
Species: Hu
FGF acidic/FGF1
[Unconjugated]
232-FA
FGF acidic/FGF1 [Unconjugated]
(1 Review)
(17 Publications)
Species: Hu
FGFR4 Antibody
[Unconjugated]
AF2265
Western blot shows lysates of mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF R4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF R4 at approximately 89 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FGF R4 was detected in immersion fixed frozen sections of mouse embryo (11.5 d.p.c.) using Goat Anti-Mouse FGF R4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2265) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(1 Review)
(2 Publications)
Species: Mu
Applications: WB, IHC
p53 Antibody (PAb
240)
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
(3 Reviews)
(23 Publications)
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
p16INK4a/CDKN2A
Antibody
NB200-106
Western Blot: p16INK4a/CDKN2A Antibody [NB200-106] - Analysis of p16INK4a/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
Immunohistochemistry-Paraffin: p16INK4a/CDKN2A Antibody [NB200-106] - Analysis of FFPE mouse spleen using p16INK4a/CDKN2A antibody at 10 ug/mL on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining in the germinal centers of spleen was observed. Staining was performed by Histowiz.
(1 Review)
(41 Publications)
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
FGF-9
[Unconjugated]
273-F9
FGF-9 [Unconjugated]
(26 Publications)
Species: Hu
ERK2 Antibody
(SZ25-01)
NBP2-67360
Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Cyclin D1 Antibody
(SPM587)
NBP2-32840
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Western Blot of cyclin D1 in (1) C2C12 (2) HepG2 & (3) NIH3T3 Lysate with Cyclin D1 Ab (SPM587)
Immunohistochemistry-Paraffin: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Formalin-paraffin human Mantle Cell Lymphoma stained with Cyclin D1 Ab (Clone SPM587).
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - analysis of Cyclin D1 in human breast cancer cell line MDA-MB-231 cell lysate using anti-Cyclin D1. Image from verified customer review.
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
ERK1 Antibody (1E5)
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
WHSC1 Antibody
(CL1063)
NBP2-52963
Western Blot: WHSC1 Antibody (CL1063) [NBP2-52963] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human colorectal cancer shows strong nuclear immunoreactivity in a subset of tumor cells.
Immunohistochemistry-Paraffin: WHSC1 Antibody (CL1063) [NBP2-52963] - Staining of human cerebral cortex shows moderate positivity in neuronal cell nuclei.
Species: Hu
Applications: WB, IHC, IHC-P
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).
(13 Publications)
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Recombinant Human
FGF-13 Protein
NBP2-35009
SDS-Page: Human FGF-13 Protein [NBP2-35009]
(2 Publications)
Species: Hu
FGF-3 Antibody
(MSD1)
NB200-603
FGF-3 Antibody (MSD1)
(1 Review)
(1 Publication)
Species: Hu, Mu, Xp
Applications: WB, IHC, IHC-Fr, IHC-P, IP, IF
KGF/FGF-7
[Unconjugated]
251-KG
KGF/FGF-7 [Unconjugated]
(42 Publications)
Species: Hu
Twist-1 Antibody
(10E4E6)
NBP2-37364
Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.
Immunocytochemistry/Immunofluorescence: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of Hela cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: Twist-1 Antibody (10E4E6) [NBP2-37364] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TWIST1 mouse mAb with DAB staining.
(2 Publications)
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-IC
FGF-8
[Unconjugated]
423-F8/CF
Recombinant Human/Mouse FGF-8b (Catalog # 423-F8/CF) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.
1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.
(42 Publications)
Species: Hu, Mu
PTHLH/PTHrP
Antibody
NBP1-59322
Western Blot: PTHLH/PTHrP Antibody [NBP1-59322] - HepG2 cell lysate, Antibody Titration: 2.0ug/ml
Immunohistochemistry-Paraffin: PTHLH/PTHrP Antibody [NBP1-59322] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.
Western Blot: PTHLH/PTHrP Antibody [NBP1-59322] - Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Goat anti-rabbit HRP Secondary, Antibody Dilution: 1 : 2000.
Species: Hu, Mu, Rt, Bv, Ca, Eq, Gt, GP, Rb
Applications: WB, IHC, IHC-P
FGFR3 Antibody
(2H10B4)
NBP2-52468
FGFR3 Antibody (2H10B4)
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready

Publications for FGFR3 Antibody (NBP2-52468)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


Filter By Application
IHC-P
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for FGFR3 Antibody (NBP2-52468) (0)

There are no reviews for FGFR3 Antibody (NBP2-52468). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FGFR3 Antibody (NBP2-52468) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

FGFR3 Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional FGFR3 Products

Bioinformatics Tool for FGFR3 Antibody (NBP2-52468)

Discover related pathways, diseases and genes to FGFR3 Antibody (NBP2-52468). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FGFR3 Antibody (NBP2-52468)

Discover more about diseases related to FGFR3 Antibody (NBP2-52468).
 

Pathways for FGFR3 Antibody (NBP2-52468)

View related products by pathway.

PTMs for FGFR3 Antibody (NBP2-52468)

Learn more about PTMs related to FGFR3 Antibody (NBP2-52468).
 

Research Areas for FGFR3 Antibody (NBP2-52468)

Find related products by research area.

Blogs on FGFR3

There are no specific blogs for FGFR3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FGFR3 Antibody (2H10B4) and receive a gift card or discount.


Bioinformatics

Gene Symbol FGFR3