FGFR1 Antibody (133111) - (IIIb)

Recombinant Human FGF R1 alpha (IIIb) Fc Chimera (Catalog # 655-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R1 alpha (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R1 (IIIb) Monoclonal Antibody (Catalog # MAB765). The ND50 is typically 0.075-0.3 µg/mL in the presence of heparin (10 µg/mL).

Proximity between AcSDKP and FGFR1 inhibits the TGF beta /smad signaling pathway in HMVECs. (a) HMVECs were treated with N-FGFR1 (1.5 μg/ml) for 48 h with or without preincubation with AcSDKP (100 nM) for 2 h, and the proximity between AcSDKP and FGFR1 was analyzed by the Duolink In Situ Assay. For each slide, images at a × 400 original magnification were obtained from six different areas. (b and c) HMVECs were treated with TGF beta 2 (5 ng/ml) for 15 min or 48 h with or without preincubation with AcSDKP for 2 h, and the p-smad3, TGF beta R1, TGF beta R2 and FGFR1 levels were analyzed by western blot. Densitometric analysis of the p-smad3/smad3, TGF beta R1/ beta -actin, TGF beta R2/ beta -actin and FGFR1/ beta -actin levels from each group (n=6) were analyzed. (d and e) HMVECs were incubated with TGF beta 2 for 15 min or 48 h with or without preincubation with AcSDKP or its mutants (AcDSPK, AcSDKA, AcADKP) (100 nM) for 2 h. The p-smad3/smad3, TGF beta R1/ beta -actin, TGF beta R2/ beta -actin and FGFR1/ beta -actin protein levels were analyzed by western blot Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28771231), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of FGFR1 in cells from breast and bone tissue. a, FGFR mRNA transcript levels in normal and cancer cells. b, Fold change in FGFR mRNA based on expression in cancers cells relative to normal cells. c, FGFR protein levels in normal versus cancer cells. Results are from triplicate experiments and the Western blot is representative of the triplicates. (*p < 0.05) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26201468), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 133111
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

FGFR1 Antibody (133111) - (IIIb) Summary

• Immunogen: A mixture of purified isoforms of recombinant human FGF R1 extracellular domains
• Specificity: Detects IIIb isoforms of human FGF R1. In direct ELISAs and Western blots, this antibody detected IIIb but not IIIc isoforms of human FGF R1, human FGF R2, and mouse FGF R2. In direct ELISA, approximately 20% cross-reactivity was observed with recombinant human (rh) FGF R3 (IIIb). No cross-reactivity was observed with rhFGF R4 or with IIIIc isoforms of rhFGF R1, rhFGF R2, rmFGF R2, or rhFGF R3.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FGFR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.075-0.3 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGFR1 Antibody (133111) - (IIIb)

• basic fibroblast growth factor receptor 1
• BFGFR
• bFGF-R-1
• CD331 antigen
• CD331
• CEK
• EC 2.7.10
• EC 2.7.10.1
• FGF R1
• FGFBR
• FGFR1
• FGFR-1
• fibroblast growth factor receptor 1
• FLGH3
• FLJ99988
• Flt-2
• FLT2H4
• Fms-like tyrosine kinase 2
• fms-related tyrosine kinase 2
• H2
• H5
• HBGFR
• heparin-binding growth factor receptor
• hydroxyaryl-protein kinase
• KAL 2
• KAL2
• N-SAM
• OGD
• Proto-oncogene c-Fgr
• soluble FGFR1 variant 1
• soluble FGFR1 variant 2

Background

FGF R1 occurs as multiple isoforms. The extracellular domain consists of three ( alpha isoforms) or two ( beta isoforms) immunoglobulin-like domains. In addition, the membrane-proximal Ig-like domain can be encoded by alternative exons yielding (IIIb) or (IIIc) isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGFR1 Antibody (MAB765)(8)

Publications using MAB765

• R Gao, Y Wu, Q Yang, L Chen, J Chen, B Wang, Z Liu, J Jin, J Li, G Wu The Interaction of Apelin and FGFR1 Ameliorated the Kidney Fibrosis through Suppression of TGFbeta-Induced Endothelial-to-Mesenchymal Transition Oxidative Medicine and Cellular Longevity, 2023-02-04;2023(0):5012474. 2/4/2023 [PMID: 36785790]
• Gao R, Kanasaki K, Li J et al. Beta klotho is essential for the anti-endothelial mesenchymal transition effects of N-acetyl-seryl-aspartyl-lysyl-proline FEBS Open Bio. NULL [PMID: 30972974]
• D Chakrabort, V Benham, B Bullard, T Kearney, HC Hsia, D Gibbon, EY Demireva, SY Lunt, JJ Bernard Fibroblast growth factor receptor is a mechanistic link between visceral adiposity and cancer Oncogene, 2017-08-07;36(48):6668-6679. 8/7/2017 [PMID: 28783178]
• Li J, Shi S, et al. FGFR1 is critical for the anti-endothelial mesenchymal transition effect of N-acetyl-seryl-aspartyl-lysyl-proline via induction of the MAP4K4 pathway. Cell Death Dis 8/3/2017 [PMID: 28771231]
• Ling Ling, Si Kee Tan, Ting Hwee Goh, Edwin Cheung, Victor Nurcombe, Andre J. van Wijnen et al. Targeting the heparin-binding domain of fibroblast growth factor receptor 1 as a potential cancer therapy Molecular Cancer 12/1/2015 [PMID: 26201468]
• Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c. Stem Cells, 2012-07-01;30(7):1455-64. 2012-07-01 [PMID: 22495904] (Neutralization, Human)
• Evgeny A. Zemskov, Irina Mikhailenko, Elizabeth P. Smith, Alexey M. Belkin Tissue transglutaminase promotes PDGF/PDGFR-mediated signaling and responses in vascular smooth muscle cells Journal of Cellular Physiology 5/1/2012 [PMID: 21769866]
• Zemskov EA, Loukinova E, Mikhailenko I, Coleman RA, Strickland DK, Belkin AM Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase. J. Biol. Chem., 2009-04-22;284(24):16693-703. 2009-04-22 [PMID: 19386600] (Bioassay, Human)

FAQs for FGFR1 Antibody (MAB765). (Showing 1 - 2 of 2 FAQs).

1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
   • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
   • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

Bioinformatics

• Gene Symbol: FGFR1