FGF-6 Antibody

Images

 
Recombinant Human FGF‑6 (Catalog # 238-F6) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑6 (1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

FGF-6 Antibody Summary

Immunogen
E. coli-derived recombinant human FGF-6
Gly41-Ile208
Accession # P10767
Specificity
Detects human FGF-6 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human (rh) FGF-4 is observed, less than 5% cross-reactivity with rhFGF-9 is observed, and less than 2% cross-reactivity with FGF-acidic, rhFGF-5, and rhFGF-7 is observed. In direct ELISAs, less than 1% cross-reactivity with rh beta -ECGF, recombinant mouse (rm) FGF-8b, and rmFGF-8c is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FGF6
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Neutralization 0.2-1 ug/mL
Publications
Read Publications using
AF238 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-6 Antibody

  • FGF6
  • FGF-6
  • fibroblast growth factor 6
  • HBGF-6
  • Heparin secretory-transforming protein 2
  • Heparin-binding growth factor 6
  • HST2
  • HST-2
  • HSTF2
  • HSTF-2

Background

Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25-28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature human FGF-6 is a 171 aa protein that shares 94% aa sequence identity with mouse and rat FGF-6 (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5‑7). FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma (7-9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).
  1. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Marics, I. et al. (1989) Oncogene 4:335.
  4. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  5. Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
  6. Asada, M. et al. (1999) Growth Factors 16:293.
  7. Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
  8. de Lapeyriere, O. et al. (1993) Development 118:601.
  9. Li, J. et al. (1993) Cancer 72:2253.
  10. Floss, T. et al. (1997) Genes Dev. 11:2040.
  11. Grass, S. et al. (1996) Development 122:141.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

    Customers Who Viewed This Item Also Viewed...

    NB100-1923
    Species: Ce
    Applications: WB, IHC, IHC-P, IP
    AF2265
    Species: Mu
    Applications: WB, IHC
    NB200-603
    Species: Hu, Mu, Xp
    Applications: WB, IHC, IHC-Fr, IHC-P, IP, IF
    NB100-2080
    Species: Hu, Mu
    Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
    NB100-56510
    Species: Hu, Mu, Rt
    Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
    AF-237-NA
    Species: Hu
    Applications: WB, IHC, Neut
    NBP2-00932
    Species: Hu
    Applications: WB, Flow, ICC/IF, IHC, IHC-P
    NBP2-44245
    Species: Hu
    Applications: WB, IHC, IHC-P
    AF-237-NA
    Species: Hu
    Applications: WB, IHC, Neut
    H00003347-M01
    Species: Hu
    Applications: ELISA, ICC/IF
    NB100-56511
    Species: Hu, Mu, Rt, Fe
    Applications: WB, ICC/IF, IHC, IHC-Fr, IP
    NBP2-32604
    Species: Hu
    Applications: WB, ICC/IF, IHC, IHC-P
    NBP2-66840
    Species: Hu, Mu, Rt
    Applications: WB, Flow, ICC/IF, IHC, IHC-P
    AF238
    Species: Hu
    Applications: WB, B/N

    Publications for FGF-6 Antibody (AF238)(2)

    We have publications tested in 1 confirmed species: Mouse.

    We have publications tested in 2 applications: IHC, WB.


    Filter By Application
    IHC
    (1)
    WB
    (1)
    All Applications
    Filter By Species
    Mouse
    (2)
    All Species

    Reviews for FGF-6 Antibody (AF238) (0)

    There are no reviews for FGF-6 Antibody (AF238). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
    • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
    • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
    • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol

    FAQs for FGF-6 Antibody (AF238) (0)

    There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

    Secondary Antibodies

     

    Isotype Controls

    Additional Array Products

    Bioinformatics Tool for FGF-6 Antibody (AF238)

    Discover related pathways, diseases and genes to FGF-6 Antibody (AF238). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
    Visit Tool

    Diseases for FGF-6 Antibody (AF238)

    Discover more about diseases related to FGF-6 Antibody (AF238).
     

    Pathways for FGF-6 Antibody (AF238)

    View related products by pathway.

    PTMs for FGF-6 Antibody (AF238)

    Learn more about PTMs related to FGF-6 Antibody (AF238).

    Blogs on FGF-6

    There are no specific blogs for FGF-6, but you can read our latest blog posts.

    Customers Who Bought This Also Bought

    Contact Information

    Product PDFs

    Calculators

    Concentration Calculator

    The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

    =
    ÷

    Review this Product

    Be the first to review our FGF-6 Antibody and receive a gift card or discount.

    Bioinformatics

    Gene Symbol FGF6
    Entrez
    Uniprot