FGF-6 Antibody

Recombinant Human FGF‑6 (Catalog # 238-F6) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF238). The ND50 is typically 0.2-1 µg/mL in the presence of heparin (1 µg/mL).

• Reactivity: Hu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

FGF-6 Antibody Summary

• Immunogen: E. coli-derived recombinant human FGF-6
  Gly41-Ile208
  Accession # P10767
• Specificity: Detects human FGF-6 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human (rh) FGF-4 is observed, less than 5% cross-reactivity with rhFGF-9 is observed, and less than 2% cross-reactivity with FGF-acidic, rhFGF-5, and rhFGF-7 is observed. In direct ELISAs, less than 1% cross-reactivity with rh beta -ECGF, recombinant mouse (rm) FGF-8b, and rmFGF-8c is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FGF6
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.2-1 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGF-6 Antibody

• FGF6
• FGF-6
• fibroblast growth factor 6
• HBGF-6
• Heparin secretory-transforming protein 2
• Heparin-binding growth factor 6
• HST2
• HST-2
• HSTF2
• HSTF-2

Background

Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25-28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature human FGF-6 is a 171 aa protein that shares 94% aa sequence identity with mouse and rat FGF-6 (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5‑7). FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma (7-9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).

1. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
3. Marics, I. et al. (1989) Oncogene 4:335.
4. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
5. Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
6. Asada, M. et al. (1999) Growth Factors 16:293.
7. Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
8. de Lapeyriere, O. et al. (1993) Development 118:601.
9. Li, J. et al. (1993) Cancer 72:2253.
10. Floss, T. et al. (1997) Genes Dev. 11:2040.
11. Grass, S. et al. (1996) Development 122:141.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FGF-6 Antibody (AF238)(2)

Publications using AF238

• Israeli D, Benchaouir R, Ziaei S, Rameau P, Gruszczynski C, Peltekian E, Danos O, Garcia L FGF6 mediated expansion of a resident subset of cells with SP phenotype in the C2C12 myogenic line. J. Cell. Physiol., 2004-12-01;201(3):409-19. 2004-12-01 [PMID: 15389547] (Western Blot, Mouse)
• Zhao P, Hoffman EP Embryonic myogenesis pathways in muscle regeneration. Dev. Dyn., 2004-02-01;229(2):380-92. 2004-02-01 [PMID: 14745964] (IHC, Mouse)

Bioinformatics

• Gene Symbol: FGF6
• Uniprot:
  • P10767()