Ferroportin/SLC40A1 Antibody [Alexa Fluor® 700]

Images

 
Flow Cytometry: Ferroportin/SLC40A1 Antibody [Alexa Fluor (R) 700] [NBP1-21502AF700] - Histogram of Ferroportin staining of human spleen macrophages (red). Image from verified customer review.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 700

Ferroportin/SLC40A1 Antibody [Alexa Fluor® 700] Summary

Immunogen
Synthetic peptide made to an internal portion of human Ferroportin 1 (within residues 250-300). [Swiss-Prot# Q9NP59]
Localization
Cell membrane (high), Cytoplasm (low)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC40A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Ferroportin 1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~62 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-21502AF700 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Ferroportin/SLC40A1 Antibody [Alexa Fluor® 700]

  • Ferroportin
  • Ferroportin-1
  • FPN1
  • FPN1IREG1ferroportin 1
  • HFE4
  • HFE4ferroportin-1
  • IREG1
  • Iron-regulated transporter 1
  • member 3
  • MST079
  • MTP1
  • putative ferroportin 1 variant IIIB
  • SLC11A3
  • SLC11A3iron regulated gene 1
  • SLC40A1
  • solute carrier family 11 (proton-coupled divalent metal ion transporters)
  • solute carrier family 40 (iron-regulated transporter), member 1
  • solute carrier family 40 member 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ferroportin/SLC40A1 Antibody (NBP1-21502AF700) (0)

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Review for Ferroportin/SLC40A1 Antibody (NBP1-21502AF700) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NBP1-21502AF700:
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Images Ratings Applications Species Date Details
Flow Cytometry Ferroportin/SLC40A1 NBP1-21502AF700
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reviewed by:
Anonymous
Flow Human 08/24/2016
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Summary

ApplicationFlow Cytometry
Sample Testedspleen
SpeciesHuman
LotC-4-072216-AF700

Blocking

Blocking Details1% MOUSE SERUM, 1% RAT SERUM AND 5% HUMAN SERUM

Details

Detection NotesCELLS WERE STAINED FOR 30MINS. DILUTE ANTIBODY 1:100. NEGATIVE CONTROL: ISOTYPE CONTROL.

Comments

CommentsOPTIMAL WORKING CONCENTRATION WAS FOUND TO BE 7.5 MICROGRAM PER ML. I.E. DILUTE 1:100

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ferroportin/SLC40A1 Antibody (NBP1-21502AF700) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Ferroportin/SLC40A1 Products

Bioinformatics Tool for Ferroportin/SLC40A1 Antibody (NBP1-21502AF700)

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Recent Reviews

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5
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2
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1
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Anonymous
08/24/2016
Application: Flow
Species: Human

Bioinformatics

Gene Symbol SLC40A1
Entrez