Ferritin Light Chain Antibody (X1) - Azide and BSA Free

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M16), clone X1. Lane 1: FTL transfected lysatE (20 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of monoclonal antibody to FTL on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of FTL expression in K-562 (Cat # L009V1).

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of FTL transfected lysate using anti-FTL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FTL rabbit polyclonal antibody.

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of FTL over-expressed 293 cell line, cotransfected with FTL Validated Chimera RNAi ( Cat # H00002512-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FTL monoclonal antibody (M16), clone X1 (Cat # H00002512-M16 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Sandwich ELISA: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Detection limit for recombinant GST tagged FTL is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP, KD
• Clone: X1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Ferritin Light Chain Antibody (X1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: FTL (AAH04245, 1 a.a. ~ 175 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FTL
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot
• Application Notes: It has been used for ELISA, WB, IP and IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Ferritin Light Chain Antibody (X1) - Azide and BSA Free

• Ferritin L subunit
• ferritin L-chain
• ferritin light chain
• ferritin light polypeptide-like 3
• ferritin, light polypeptide
• MGC71996
• NBIA3

Background

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ferritin Light Chain Antibody (H00002512-M16)(1)

Publication using H00002512-M16

• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01

Bioinformatics

• Gene Symbol: FTL
• Uniprot:
  • AAH04245(Human)