Fer Antibody (5D2C4)

Images

 
Western Blot: Fer Antibody (5D2C4) [NBP1-47506] - Analysis using FER mouse mAb against NIH/3T3 (1), A549 (2) and SK-MEL-5 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Fer Antibody (5D2C4) [NBP1-47506] - Analysis of Hela cells using FER mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 ...read more
Immunohistochemistry-Paraffin: Fer Antibody (5D2C4) [NBP1-47506] - Analysis of human kidney tissues using FER mouse mAb.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
5D2C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Fer Antibody (5D2C4) Summary

Immunogen
Purified recombinant fragment of human FER expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FER
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Fer Antibody (5D2C4)

  • EC 2.7.10
  • EC 2.7.10.2
  • fer (fps/fes related) tyrosine kinase
  • Fer
  • p94-Fer
  • Phosphoprotein NCP94
  • Proto-oncogene c-Fer
  • TYK3
  • TYK3phosphoprotein NCP94
  • Tyrosine kinase 3
  • tyrosine-protein kinase Fer

Background

Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells (1). FER is found in the cytoplasm and in the nucleus, where it was associated with the chromatin fraction. Its ubiquitous expression and its subcellular location indicate that it may be involved in key regulatory processes (2). The group IV cytoplasmic protein-tyrosine kinase Fer has been linked to cellular signaling responses to many different stimuli, including growth factors and cytokines. Data suggest a biological role for Fer in regulation of leukocyte recruitment during the innate immune response (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fer Antibody (NBP1-47506). (Showing 1 - 1 of 1 FAQ).

  1. I have recently purchased an antibody from you: NBP1-47506, Fer antibody (5D2C4). Could you tell me the epitope it is raised to please?
    • The exact sequence is considered proprietary, however the sequence falls between amino acids 280-474. This antibody has not been epitope mapped as far as we know.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FER
Entrez
Uniprot