FEM1A Antibody

Western Blot: FEM1A Antibody [NBP1-36956] - (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FEM1A Antibody Summary

• Description: Novus Biologicals Goat FEM1A Antibody (NBP1-36956) is a polyclonal antibody validated for use in WB and ELISA. Anti-FEM1A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-GFIPEDLEAFIELH corresponding to C-Terminus according to NP_061178.1.
• Epitope: C Terminus
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FEM1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 73.6 kDa band observed in human heart lysates (calculated MW of 73.6 kDa band according to NP_061178.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FEM1A Antibody

• DKFZp762M136
• EPRAP
• fem-1 homolog a (C. elegans)
• FEM1a
• FEM1-alpha
• Prostaglandin E receptor 4-associated protein
• protein fem-1 homolog A

Background

FEM1A is a caspase substrate homologous to the C. Elegans sex determining protein, FEM-1. Over expression of FEM1A in mammalian cells is reported to induce apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FEM1A Antibody (NBP1-36956)(1)

Publication using NBP1-36956

• Minami M, Shimizu K, Okamoto Y et al. Prostaglandin E receptor type 4-associated protein interacts directlywith NF-kappaB1 and attenuates macrophage activation. J Biol Chem 2008-04-11 [PMID: 18270204]

Bioinformatics

• Gene Symbol: FEM1A
• Entrez:
  • 55527(Human)
• Uniprot:
  • NP_061178.1(Human)