FECH Overexpression Lysate

Images

 
Western Blot: FECH Overexpression Lysate (Adult Normal) [NBL1-10673] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FECH.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FECH Overexpression Lysate Summary

Description

FECH Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC208058

Accession#: NM_000140

Protein Tag: C-MYC/DDK
Gene
FECH

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
42.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FECH Overexpression Lysate

  • EC 4.99.1.1
  • EPP
  • FCE
  • ferrochelatase (protoporphyria)
  • ferrochelatase
  • ferrochelatase, mitochondrial
  • Heme synthase
  • heme synthetase
  • Protoheme ferro-lyase
  • protoporphyria

Background

The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) uses an alternate in-frame donor splice site at one of the internal coding exons, compared to transcript variant 1. This results in a slightly shorter isoform (b) with the same N- and C- termini, but missing a 6 aa segment compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for FECH Lysate (NBL1-10673) (0)

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Bioinformatics

Gene Symbol FECH