FCRN/FCGRT Overexpression Lysate (Native)

Images

 
Western Blot: FCGRT Overexpression Lysate (Adult Normal) [NBL1-10660] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FCGRT.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

FCRN/FCGRT Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200364 and based on accession number NM_004107. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Fc fragment of IgG, receptor, transporter, alpha (FCGRT), transcript variant 1, mRNA*
Gene
FCGRT

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
39.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for FCRN/FCGRT

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FCRN/FCGRT Overexpression Lysate (Native)

  • alpha-chain
  • Fc fragment of IgG, receptor, transporter, alpha
  • FCGRT
  • FcRn alpha chain
  • FCRN
  • FCRNimmunoglobulin receptor, intestinal, heavy chain
  • IgG Fc fragment receptor transporter alpha chain
  • IgG receptor FcRn large subunit p51
  • major histocompatibility complex class I-like Fc receptor
  • Neonatal Fc receptor
  • neonatal Fc-receptor for Ig

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for FCRN/FCGRT Lysate (NBL1-10660) (0)

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Product General Protocols

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Video Protocols

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FAQs for FCRN/FCGRT Lysate (NBL1-10660). (Showing 1 - 1 of 1 FAQ).

  1. Can you please confirm whether the following items are both related to neonatal Fc receptor? NBL1-10660 & H00002217-Q01
    • Both of these products are of the Fc fragment of IgG, receptor, transporter, alpha (FCGRT). One is an overexpression lysate and the other is a purified partial recombinant protein. Our description of the gene is as follows This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. This appears to be the neonatal Fc receptor.

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Bioinformatics Tool for FCRN/FCGRT Lysate (NBL1-10660)

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Blogs on FCRN/FCGRT.

FcRn - neonatal Fc receptor encoded by the FCGRT gene
Antibodies play an important role in the innate immune system by circulating in the bloodstream to fight off invading pathogens. IgG is the most prevalent of the five classes of antibodies (IgA, IgD, IgE, IgG, and IgM) and is the only one transmit...  Read full blog post.

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Bioinformatics

Gene Symbol FCGRT
Entrez