FCRL3/FcRH3 Antibody

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Human peripheral blood lymphocytes were stained with Goat Anti-Human FCRL3/FcRH3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3126) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

FCRL3/FcRH3 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human FCRL3/FcRH3
Arg14-Arg569
Accession # NP_443171
Specificity
Detects human FCRL3/FcRH3 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) IRTA2 and rhIRTA5 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FCRL3
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FCRL3/FcRH3 Antibody

  • CD307c
  • Fc receptor homolog 3
  • Fc receptor-like 3
  • Fc receptor-like protein 3
  • FcRH3
  • FCRL3
  • fcR-like protein 3
  • hIFGP3
  • IFGP3
  • immunoglobulin superfamily receptor translocation associated protein 3
  • IRTA3
  • SPAP2

Background

FCRL3 (Fc Receptor-Like 3), also known as FcRH3, IRTA3, and SPAP2, is a 110 kDa molecule with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1). Mature human FCRL3 consists of a 556 amino acid (aa) extracellular domain (ECD) with six Ig-like domains, a 21 aa transmembrane segment, and a 140 aa cytoplasmic domain with four immunotyrosine inhibitory motifs (ITIMs) (2 - 4). Within the ECD, human and mouse FCRL3 share 35% aa sequence identity. Alternate splicing generates several additional isoforms with deletions or substitutions in both the extracellular and intracellular regions. These include potentially secreted forms that are truncated following the second Ig-like domain (4). FCRL3 is expressed in secondary lymphoid organs on the surface of mature naïve and memory B cells, NK cells, and B cell lines derived from chronic lymphocytic leukemias (2, 3, 5). It is upregulated on B cells following LPS or anti-CD40 stimulation (6). A polymorphism in the FCRL3 promoter induces enhanced transcription and is associated with the development of autoimmune disorders in a Japanese population (6, 7). Tyrosine phosphorylation within the ITIMs of FCRL3 enables its association with SHP-1 (4).

  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Miller, I. et al. (2002) Blood, 99:2662.
  3. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
  4. Xu, M.-J. et al. (2002) Biochem. Biophys. Res. Commun. 293:1037.
  5. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  6. Kochi, Y. et al. (2005) Nat. Genet. 37:478.
  7. Chistiakov, D.A. and A.P. Chistiakov (2007) Hum. Immunol. 68:375.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FCRL3
Uniprot