FCRL2/FcRH2 Antibody (296902) [Allophycocyanin]

• Applications: Flow
• Clone: 296902
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

FCRL2/FcRH2 Antibody (296902) [Allophycocyanin] Summary

• Additional Information: Clone 296902 was used by HLDA to establish CD designation
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human FCRL2/FcRH2
  Glu15-Asp395
  Accession # Q96LA5
• Specificity: Detects human FCRL2/FcRH2 and FCRL5/FcRH5 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human FCRL1, FCRL3, and FCRL4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCRL2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Fc Receptor-Like 2 (FCRL2), also known as FcRH2 and IRTA4, belongs to the family of glycoprotein homologs of classical immunoglobulin (Ig) Fc receptors. In human, the type I transmembrane FCRL protein family contains from three to nine immunoglobulin-like domains (1, 2). Mature human FcRH2 consists of a 382 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-set domains, a 21 aa transmembrane segment, and an 86 aa cytoplasmic domain with one ITAM-like, and two ITIM-like motifs (3-5). Alternate splicing of human FCRL2 may generate isoforms with N-terminal, internal, or C-terminal deletions (4, 5). The gene for FcRH2 maps to the human Iq21-23 locus which is a hotspot for chromosomal translocation events associated with B cell malignancies (3, 6). Although there are several Fc receptor-like genes in the mouse, none of these is a clear ortholog to human FCRL2 (7). FCRL proteins are differentially expressed among B cells (2). FCRL2 is preferentially expressed on naïve and CD27⁺ memory B cells within the spleen, lymph nodes, tonsils, and peripheral blood (3, 4, 8, 9). It is also expressed on most B cells in B cell chronic lymphocytic leukemia (B-CLL) patients (10). FCRL2 upregulation is associated with mutation of the Immunoglobulin Heavy Chain Variable (IGHV) and less aggressive forms of B-CLL (9, 11).

 

1. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
2. Davis, R.S. et al. (2007) Annu. Rev. Immunol. 25:525.
3. Miller, I. et al. (2002) Blood 99:2662.
4. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
5. Xu, M.J. et al. (2001) Biochem. Biophys. Res. Commun. 280:768.
6. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
7. Davis, R.S. et al. (2004) Int. Immunol. 16:1343.
8. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
9. Huttmann, A. et al. (2006) Leukemia 20:1774.
10. Kazemi, T. et al. (2008) Int. J. Cancer 123:2113.
11. Li, F.J. et al. (2008) Blood 112:179.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCRL2