FCRL1/FcRH1 Antibody (745916)

Images

 
Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat IgG1 Isotype Control (Catalog # MAB005) or (B) Rat Anti-Mouse FCRL1/FcRH1 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
745916
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

FCRL1/FcRH1 Antibody (745916) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FCRL1/FcRH1
Ala17-Ser219
Accession # Q8R4Y0
Specificity
Detects mouse FCRL1/FcRH1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FCRL1/FcRH1 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
FCRL1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FCRL1/FcRH1 Antibody (745916)

  • BXMH1
  • CD307a antigen
  • CD307a
  • Fc receptor homolog 1
  • Fc receptor-like 1
  • FcRH1
  • FCRH1DKFZp667O1421
  • FCRL1
  • FcR-like protein 1
  • hIFGP1
  • IFGP family protein 1
  • IFGP1
  • IFGP1Fc receptor-like protein 1
  • Immune receptor translocation-associated protein 5
  • IRTA5
  • IRTA5immunoglobulin superfamily Fc receptor, gp42

Background

FCRL1 (Fc receptor-like protein 1; also CD307a, FcRH1 and IFGP1) is a 36 kDa (predicted) member of the Ig Superfamily. In mouse, it is found on mature follicular and marginal B cells, splenic T cells and NK cells. It would appear that mouse FCRL1 serves as an inhibiting coreceptor, in as much as it contains a series of cytoplasmic ITIM motifs. Mature mouse FCRL1 is a 327 amino acid (aa) type I transmembrane protein (aa 17-343). It contains a 203 aa extracellular region (aa 17‑219) that shows two C2-type Ig-like domains (aa 17-200), and a 103 aa cytoplasmic domain. There are multiple potential splice variants. One shows a deletion of aa 206-248 (potentially soluble), a second contains a six aa substitution for aa 319-343, a third possesses a three aa substitution for aa 209-343, and a fourth shows a 21 aa substitution for Ala11. Over aa 17-219, mouse FCRL1 shares 83% aa sequence identity with rat FCRL1. Over the same region, it shares 63% aa sequence identity with human FCRL1 when a third Ig-like domain that is present in human is excluded from the analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FCRL1
Uniprot