FCER1G Antibody - BSA Free

Immunohistochemistry-Paraffin: FCER1G Antibody [NBP2-16456] - Immunohistochemical analysis of paraffin-embedded U873 xenograft, using antibody at 1:100 dilution.

ICC/IF-FCER1G Antibody [NBP2-16456] -HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FCER1G stained by FCER1G antibody [N2C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10um.

Western Blot: FCER1G Antibody [NBP2-16456] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: FCER1G Antibody [NBP2-16456] - Non-transfected - and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [N2C3]diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

FCER1G Antibody [NBP2-16456] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FCER1G stained by FCER1G antibody [N2C3] diluted at 1:500.Blue: Fluoroshield with DAPI.

Western Blot: FCER1G Antibody [NBP2-16456] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [N2C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Po, Ca, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

FCER1G Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FCER1G Antibody - BSA Free (NBP2-16456) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FCER1G. The exact sequence is proprietary.
• Localization: Cell membrane; Single-pass type I membrane protein
• Predicted Species: Rat (100%), Porcine (100%), Rhesus Macaque (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FCER1G
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 10 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for FCER1G Antibody - BSA Free

• Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
• Fc-epsilon RI-gamma
• FceRI gamma
• FCRG
• high affinity immunoglobulin epsilon receptor subunit gamma
• IgE Fc receptor subunit gamma
• immunoglobulin E receptor, high affinity, gamma chain

Background

The high affinity IgE receptor is a key molecule involved in allergic reactions. It is a tetramer composed of 1 alpha, 1 beta, and 2 gamma chains. The gamma chains are also subunits of other Fc receptors. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: FCER1G
• Uniprot:
  • P30273(Human)