FCAR/CD89 Antibody (14A8 (14.1)) - Chimeric - Azide and BSA Free

Flow Cytometry: FCAR/CD89 Antibody (14A8 (14.1)) - Chimeric - Azide and BSA Free [NBP3-20161] - Paraformaldehyde fixed human peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 14A8 (14.1) (NBP3-20161, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: ELISA, Flow, IP
• Clone: 14A8 (14.1)
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

FCAR/CD89 Antibody (14A8 (14.1)) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit FCAR/CD89 Antibody (14A8 (14.1)) - Chimeric - Azide and BSA Free (NBP3-20161) is a recombinant monoclonal antibody validated for use in ELISA, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: The original antibody was generated by immunizing HCO12 trangene containing mice with recombinant soluble CD89 antigen.
• Epitope: This antibody binds CD89 in the extracellular domain 2, outside the IgA-binding site.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FCAR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. It binds human CD89 with a dissociation constant of Kd= 6.8 X10-10M.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for FCAR/CD89 Antibody (14A8 (14.1)) - Chimeric - Azide and BSA Free

• CD89 antigen
• CD89
• CD89IgA Fc receptor
• Fc alpha receptor
• Fc fragment of IgA, receptor for
• FCAR
• immunoglobulin alpha Fc receptor

Background

The CD89 gene is a member of the immunoglobulin gene superfamily and encodes a receptor for the Fc region of IgA. Thereceptor is a transmembrane glycoprotein present on the surface of myeloid lineage cells such as neutrophils,monocytes, macrophages, and eosin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCAR