Fc gamma RII/CD32 Antibody (3D3) - BSA Free

Mass cytometry (surface staining) of human peripheral blood with anti-CD32 (3D3) 167Er. Gated on singlets.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 3D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Fc gamma RII/CD32 Antibody (3D3) - BSA Free Summary

• Description: Novus Biologicals Mouse Fc gamma RII/CD32 Antibody (3D3) - BSA Free (NBP3-09138) is a monoclonal antibody validated for use in Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified glycosylated recombinant human FcgammaRIIa2
• Specificity: The mouse monoclonal antibody 3D3 recognizes an extracellular epitope of CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 isoforms on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCGR2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2-6 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Fc gamma RII/CD32 Antibody (3D3) - BSA Free

• CD32 antigen
• CD32B
• CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
• CDw32
• Fc fragment of IgG, low affinity IIb, receptor (CD32)
• Fc fragment of IgG, low affinity IIb, receptor for (CD32)
• Fc gamma RII
• Fc gamma RIIb
• FCG2
• Fc-gamma RII-b
• FcgammaRIIb
• fc-gamma-RIIb
• FCGR2
• FcgRII
• FcgRIIB
• fcRII-b
• FcRII-c
• IGFR2
• IgG Fc receptor II-b
• low affinity immunoglobulin gamma Fc region receptor II-b

Background

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCGR2B