Fc gamma RI/CD64 Antibody (H22) [CoraFluor™ 1]

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF
• Clone: H22
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: CoraFluor 1

Fc gamma RI/CD64 Antibody (H22) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human Fc gamma RI/CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A.
• Specificity: This immunotoxin is specific for human Fc gamma RI/CD64, and specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by fusion of the m22(scFv) coding regions to the truncated ETA coding sequences
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FCGR1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA Validated for Competitive ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Fc gamma RI/CD64 Antibody (H22) [CoraFluor™ 1]

• CD64 antigen
• CD64
• CD64a
• Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Fc gamma RI
• FCG1
• Fc-gamma receptor I B2
• Fc-gamma RI
• Fc-gamma RIA
• FcgammaRIa
• FCGR1
• FcgRI
• FcgRIA
• FCRI
• FcRIA
• FLJ18345
• high affinity Ia, receptor for (CD64)
• high affinity immunoglobulin gamma Fc receptor I
• IgG Fc receptor I

Background

CD64 is a 72 kD single chain type I glycoprotein also known as Fc gammaRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN- gamma stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FCGR1A