Fc gamma RI/CD64 Antibody (10.1) [Unconjugated]

Human peripheral blood monocytes were stained with Mouse Anti-Human Fc gamma RI/CD64 Monoclonal Antibody (Catalog # MAB1257, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Fc gamma RI/CD64 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human Fc gamma RI/CD64 Monoclonal Antibody (Catalog # MAB1257) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 10.1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Fc gamma RI/CD64 Antibody (10.1) [Unconjugated] Summary

• Additional Information: Clone 10.1 was used by HLDA to establish CD designation
• Immunogen: Rheumatoid synovial fluid cells and fibronectin purified monocytes
• Specificity: Detects human Fc gamma RI/CD64.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: FCGR1A

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RI/CD64 Antibody (10.1) [Unconjugated]

• CD64 antigen
• CD64
• CD64a
• Fc fragment of IgG, high affinity Ia, receptor (CD64)
• Fc gamma RI
• FCG1
• Fc-gamma receptor I B2
• Fc-gamma RI
• Fc-gamma RIA
• FcgammaRIa
• FCGR1
• FcgRI
• FcgRIA
• FCRI
• FcRIA
• FLJ18345
• high affinity Ia, receptor for (CD64)
• high affinity immunoglobulin gamma Fc receptor I
• IgG Fc receptor I

Background

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10^‑8 ‑ 10^‑9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10^‑6 ‑ 10^‑7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Three highly homologous genes (A, B, and C) sharing 98% identity at the nucleotide level have been identified for the human CD64 group (1). Fc gamma RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associatedFc R gamma accessory chain. The genes for Fc gamma  RIB and Fc gamma  RIC contain stop codons within their membrane proximal Ig-like domains indicating possible secreted receptors (1, 6). An mRNA splice variant of Fc gamma RIB has a deletion of the membrane-proximal Ig-like domain and encodes a putative transmembrane receptor (6). The high affinity recognition of IgG by Fc gamma RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up‑regulated during bacterial infections and sepsis.

1. Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
5. Ravetch, J. and L. Lanier (2000) Science 290:84.
6. Ernst, L. et al. (1998) Mol Immunol. 35:943.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Fc gamma RI/CD64 Antibody (MAB1257)(4)

Publications using MAB1257

• Farrukh T Awan Therapeutic CD94/NKG2A blockade improves natural killer cell dysfunction in chronic lymphocytic leukemia Oncoimmunology, 2016;5(10):e1226720. 2016 [PMID: 27853650] (Neutralization, Human)
• He X, Sun X, Wang J, Wang X, Zhang Q, Tzipori S, Feng H Antibody-enhanced, Fc gamma receptor-mediated endocytosis of Clostridium difficile toxin A. Infect Immun, 0;77(6):2294-303. 0 [PMID: 19307220]
• Schleinitz N, Cognet C, Guia S, Laugier-Anfossi F, Baratin M, Pouget J, Pelissier JF, Harle JR, Vivier E, Figarella-Branger D Expression of the CD85j (leukocyte Ig-like receptor 1, Ig-like transcript 2) receptor for class I major histocompatibility complex molecules in idiopathic inflammatory myopathies. Arthritis Rheum., 2008;58(10):3216-23. 2008 [PMID: 18821690] (IHC-Fr, Human)
• Yamaguchi Y, Seta N, Kaburaki J, Kobayashi K, Matsuura E, Kuwana M Excessive exposure to anionic surfaces maintains autoantibody response to 2-glycoprotein I in patients with antiphospholipid syndrome. Blood, 2007;110(13):4312-8. 2007 [PMID: 17726161] (Neutralization, Human)

Bioinformatics

• Gene Symbol: FCGR1A