Fc epsilon RI Antibody (43160) [Biotin]

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Flow Cytometry: Fc epsilon RI Antibody (MAR-1) [Biotin] [NBP1-43523] - Staining of MC/9 cell line with 0.06 ug of Armenian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Mouse Fc epsilon Receptor ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
43160
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Biotin
Concentration
0.5 mg/ml

Order Details

Fc epsilon RI Antibody (43160) [Biotin] Summary

Immunogen
The immunogen for this antibody was FceRI alpha.
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
FCER1A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.25 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS (pH 7.2) with 0.1% gelatin
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for Fc epsilon RI Antibody (43160) [Biotin]

  • alpha polypeptide
  • Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
  • Fc-epsilon RI-alpha
  • FcERI
  • high affinity immunoglobulin epsilon receptor alpha-subunit
  • high affinity immunoglobulin epsilon receptor subunit alpha
  • high-affinity, of mast cells, alpha polypeptide

Background

The MAR-1 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI alpha is expressed on mast and basophil cells and is up-regulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-43279AF350
Alexa Fluor 405 NBP1-43279AF405
Alexa Fluor 488 NBP1-43279AF488
Alexa Fluor 532 NBP1-43279AF532
Alexa Fluor 594 NBP1-43279AF594
Alexa Fluor 647 NBP1-43279AF647
Alexa Fluor 700 NBP1-43279AF700
Alexa Fluor 750 NBP1-43279AF750
Allophycocyanin NBP1-43279APC
Allophycocyanin/Cy7 NBP1-43279APCCY7
Biotin NBP1-43523
Biotin NBP1-43523
Biotin NBP1-43523
DyLight 350 NBP1-43279UV
DyLight 405 NBP1-43279V
DyLight 488 NBP1-43279G
DyLight 550 NBP1-43279R
DyLight 594 NBP1-43279DL594
DyLight 650 NBP1-43279C
DyLight 680 NBP1-43279FR
DyLight 755 NBP1-43279IR
FITC NBP1-43941
FITC NBP1-43941
FITC NBP1-43941
Janelia Fluor 549 NBP1-43279JF549
Janelia Fluor 646 NBP1-43279JF646
PE NBP1-43279PE
PE/Atto594 NBP1-43279PEATT594
PE/Cy5.5 NBP1-43279PECY55
PE/Cy7 NBP1-43279PECY7
PerCP NBP1-43279PCP

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Bioinformatics

Gene Symbol FCER1A