FBXW7/Cdc4 Antibody

Images

 
Immunohistochemistry: FBXW7/Cdc4 Antibody [NB100-88139] - Detection of human FBW7 by immunohistochemistry. Sample: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-FBW7 (NB100-88139). ...read more
Immunohistochemistry-Paraffin: FBXW7/Cdc4 Antibody [NB100-88139] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-FBW7 used at a dilution of 1:500. Detection: DAB staining using ...read more
Immunohistochemistry-Paraffin: FBXW7/Cdc4 Antibody [NB100-88139] - Human breast carcinoma. Antibody used at a dilution of 1:1,000.
Immunohistochemistry-Paraffin: FBXW7/Cdc4 Antibody [NB100-88139] - FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-FBW7 left and- right used at a dilution of 1:1,000 (1ug/ml). ...read more
Immunoprecipitation: FBXW7/Cdc4 Antibody [NB100-88139] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-88139 used for IP at 3 mcg/mg lysate. FBW7 was also immunoprecipitated by NB100-88138, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

FBXW7/Cdc4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human F-box and WD-40 domain protein 7 using the numbering given in entry NP_361014.1 (GeneID 55294).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FBXW7
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
FBXW7/Cdc4 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FBXW7/Cdc4 Antibody

  • AGO
  • AGOFBXO30
  • Archipelago Homolog
  • archipelago, Drosophila, homolog of
  • Cdc4
  • CDC4FLJ16457
  • F-box and WD repeat domain containing 7
  • F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila)
  • F-box and WD-40 domain-containing protein 7
  • F-box protein FBW7
  • F-box protein FBX30
  • F-box protein SEL-10
  • F-box/WD repeat-containing protein 7
  • FBW6
  • Fbw7
  • FBW7hCdc4
  • FBX30
  • FBX30FLJ11071
  • FBXO30
  • FBXW6
  • FBXW7
  • hAgo
  • homolog of C elegans sel-10
  • SCF(Fbw7)
  • SEL10
  • SEL-10DKFZp686F23254
  • SEL10FBW6

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FBXW7
Entrez
Uniprot