FBXW7/Cdc4 Overexpression Lysate (Native)


Western Blot: FBW7 Overexpression Lysate (Adult Normal) [NBL1-10647] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FBW7.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

FBXW7/Cdc4 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC217398 and based on accession number NM_033632. The protein contains a C-terminal DDK tag.
Homo sapiens F-box and WD repeat domain containing 7 (FBXW7), transcript variant 1, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
79.5 kDa

Lysate Details for FBXW7/Cdc4

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FBXW7/Cdc4 Overexpression Lysate (Native)

  • AGO
  • Archipelago Homolog
  • archipelago, Drosophila, homolog of
  • Cdc4
  • CDC4FLJ16457
  • F-box and WD repeat domain containing 7
  • F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila)
  • F-box and WD-40 domain-containing protein 7
  • F-box protein FBW7
  • F-box protein FBX30
  • F-box protein SEL-10
  • F-box/WD repeat-containing protein 7
  • FBW6
  • Fbw7
  • FBW7hCdc4
  • FBX30
  • FBX30FLJ11071
  • FBXO30
  • FBXW6
  • hAgo
  • homolog of C elegans sel-10
  • SCF(Fbw7)
  • SEL10
  • SEL-10DKFZp686F23254
  • SEL10FBW6


This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
Species: Hu, Rt
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP, RNAi, S-ELISA
Species: Hu
Applications: WB, PEP-ELISA
Species: Hu, Mu, Rt(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICFlow
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC
Species: Hu
Applications: WB
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB

Publications for FBXW7/Cdc4 Lysate (NBL1-10647) (0)

There are no publications for FBXW7/Cdc4 Lysate (NBL1-10647).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FBXW7/Cdc4 Lysate (NBL1-10647) (0)

There are no reviews for FBXW7/Cdc4 Lysate (NBL1-10647). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FBXW7/Cdc4 Lysate (NBL1-10647) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional FBXW7/Cdc4 Products

Related Products by Gene

Bioinformatics Tool for FBXW7/Cdc4 Lysate (NBL1-10647)

Discover related pathways, diseases and genes to FBXW7/Cdc4 Lysate (NBL1-10647). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FBXW7/Cdc4 Lysate (NBL1-10647)

Discover more about diseases related to FBXW7/Cdc4 Lysate (NBL1-10647).

Pathways for FBXW7/Cdc4 Lysate (NBL1-10647)

View related products by pathway.

PTMs for FBXW7/Cdc4 Lysate (NBL1-10647)

Learn more about PTMs related to FBXW7/Cdc4 Lysate (NBL1-10647).

Blogs on FBXW7/Cdc4

There are no specific blogs for FBXW7/Cdc4, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol FBXW7

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-10647 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought