FBXW7/Cdc4 Antibody - BSA Free

Immunoprecipitation: FBXW7/Cdc4 Antibody [NB100-88138] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. NB100-88138 used for IP at 6 ug per reaction. FBXW7/Cdc4 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated FBXW7/Cdc4, NB100-88138 was used at 0.1 ug/ml. Detected by chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FBXW7/Cdc4 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human F-box and WD-40 domain protein 7 using the numbering given in entry NP_361014.1 (GeneID 55294).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FBXW7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
• Control:

  FBXW7/Cdc4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FBXW7/Cdc4 Antibody - BSA Free

• AGO
• AGOFBXO30
• Archipelago Homolog
• archipelago, Drosophila, homolog of
• Cdc4
• CDC4FLJ16457
• F-box and WD repeat domain containing 7
• F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila)
• F-box and WD-40 domain-containing protein 7
• F-box protein FBW7
• F-box protein FBX30
• F-box protein SEL-10
• F-box/WD repeat-containing protein 7
• FBW6
• Fbw7
• FBW7hCdc4
• FBX30
• FBX30FLJ11071
• FBXO30
• FBXW6
• FBXW7
• hAgo
• homolog of C elegans sel-10
• SCF(Fbw7)
• SEL10
• SEL-10DKFZp686F23254
• SEL10FBW6

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FBXW7/Cdc4 Antibody (NB100-88138)(2)

Publications using NB100-88138

• Min SH, Lau AW, Lee TH et al. Negative regulation of the stability and tumor suppressor function of Fbw7 by the Pin1 prolyl isomerase. Mol Cell 2012-06-01 [PMID: 22608923]
• Bengoechea-Alonso MT, Ericsson J. The ubiquitin ligase Fbxw7 controls adipocyte differentiation by targeting C/EBPalpha for degradation. Proc Natl Acad Sci U S A 2010-06-01 [PMID: 20534483]

Bioinformatics

• Gene Symbol: FBXW7
• Entrez:
  • 55294(Human)
• Uniprot:
  • Q969H0(Human)