FBXO31 Antibody (OTI1H1) [Janelia Fluor® 525]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI1H1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

FBXO31 Antibody (OTI1H1) [Janelia Fluor® 525] Summary

• Immunogen: Full length human recombinant protein of human FBXO31(NP_079011) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FBXO31
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for FBXO31 Antibody (OTI1H1) [Janelia Fluor® 525]

• DKFZp434B027
• F-box only protein 31
• F-box protein 31
• FBX14DKFZp434J1815
• Fbx31
• FBXO14FLJ22477
• MGC15419
• MGC9527
• pp2386
• putative breast cancer tumor-suppressor
• SCF ubiquitin ligase specificity factor

Background

Belonging to the FBXO31 family, F-box only protein 31 (FBXO31) is a component of the SCF (SKP1-cullin-F-box) ubiquitin ligase complex and is involved in cyclin binding. At the protein level, FBXO31 plays a central role in G1 arrest following DNA damage. More specifically, these F-box proteins interact with SKP1 and recognize phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, thus resulting in G1 arrest. Diseases associated with FBXO31 include breast cancer, mast cell neoplasm, esophagitis and carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBXO31