FBXO31 Antibody (OTI1H1) - Azide and BSA Free

Western Blot: FBXO31 Antibody (OTI1H1) - Azide and BSA Free [NBP2-72208] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO31 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO31.

Immunocytochemistry/Immunofluorescence: FBXO31 Antibody (OTI1H1) - Azide and BSA Free [NBP2-72208] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FBXO31.

Flow Cytometry: FBXO31 Antibody (OTI1H1) - Azide and BSA Free [NBP2-72208] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-FBXO31 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI1H1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

FBXO31 Antibody (OTI1H1) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human FBXO31(NP_079011) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FBXO31
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 60.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for FBXO31 Antibody (OTI1H1) - Azide and BSA Free

• DKFZp434B027
• F-box only protein 31
• F-box protein 31
• FBX14DKFZp434J1815
• Fbx31
• FBXO14FLJ22477
• MGC15419
• MGC9527
• pp2386
• putative breast cancer tumor-suppressor
• SCF ubiquitin ligase specificity factor

Background

Belonging to the FBXO31 family, F-box only protein 31 (FBXO31) is a component of the SCF (SKP1-cullin-F-box) ubiquitin ligase complex and is involved in cyclin binding. At the protein level, FBXO31 plays a central role in G1 arrest following DNA damage. More specifically, these F-box proteins interact with SKP1 and recognize phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, thus resulting in G1 arrest. Diseases associated with FBXO31 include breast cancer, mast cell neoplasm, esophagitis and carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FBXO31