Western Blot: FANCG Antibody [NBP1-06035] - Staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FANCG Antibody [NBP1-06035] - staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: FANCG Antibody [NBP1-06035] - Analysis of FANCG using this antibody at 0.5 ug/ml in HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: FANCG Antibody [NBP1-06035] - HEK293 overexpressing FANCG and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: FANCG Antibody [NBP1-06035] - staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
WB: Approx. 70 kDa band observed in HeLa lysates (calculated MW of 68.6 kDa band according to NP_004620.1). In transfected HEK293 transiently expressing FANCG a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293. IHC: Paraffin embedded Human Uterus and Spleen.
Predicted cross-reactivity based on sequence identity: Canine, Porcine.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for FANCG Antibody
DNA repair protein XRCC9
Fanconi anemia group G protein
Fanconi anemia, complementation group G
X-ray repair, complementing defective, in Chinese hamster, 9
XRCC9X-ray repair complementing defective repair in Chinese hamster cells 9
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Fanconi Antibodies and Cancer Research We at Novus Biologicals have an extensive antibody databasedevoted to the 13 Fanconi anaemia complementation (FANC) genes, which are involved in the recognition and repair of damaged DNA.The core complex of 8 proteins (FANCA, B, C, E, F, G, L and M)... Read full blog post.
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