FANCG Antibody

Images

 
Western Blot: FANCG Antibody [NBP1-06035] - Staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FANCG Antibody [NBP1-06035] - staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: FANCG Antibody [NBP1-06035] - Analysis of FANCG using this antibody at 0.5 ug/ml in HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: FANCG Antibody [NBP1-06035] - HEK293 overexpressing FANCG and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: FANCG Antibody [NBP1-06035] - staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Po, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FANCG Antibody Summary

Immunogen
Peptide with sequence LEEFRTSLPKSCDL corresponding to C-Terminus according to NP_004620.1.
Epitope
LEEFRTSLPKSCDL
Predicted Species
Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FANCG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in HeLa lysates (calculated MW of 68.6 kDa band according to NP_004620.1). In transfected HEK293 transiently expressing FANCG a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293.
IHC: Paraffin embedded Human Uterus and Spleen.
Control
FANCG Overexpression Lysate
Publications
Read Publications using
NBP1-06035 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FANCG Antibody

  • DNA repair protein XRCC9
  • FAG
  • Fanconi anemia group G protein
  • Fanconi anemia, complementation group G
  • Protein FACG
  • X-ray repair, complementing defective, in Chinese hamster, 9
  • XRCC9X-ray repair complementing defective repair in Chinese hamster cells 9

Background

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FANCG Antibody (NBP1-06035)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for FANCG Antibody (NBP1-06035) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for FANCG Antibody (NBP1-06035)

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Blogs on FANCG.

Fanconi Antibodies and Cancer Research
We at Novus Biologicals have an extensive antibody databasedevoted to the 13 Fanconi anaemia complementation (FANC) genes, which are involved in the recognition and repair of damaged DNA.The core complex of 8 proteins (FANCA, B, C, E, F, G, L and M)...  Read full blog post.

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Bioinformatics

Gene Symbol FANCG
Entrez
Uniprot