FANCG Antibody

Western Blot: FANCG Antibody [NBP1-06035] - Staining of Jurkat nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: FANCG Antibody [NBP1-06035] - staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: FANCG Antibody [NBP1-06035] - Analysis of FANCG using this antibody at 0.5 ug/ml in HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: FANCG Antibody [NBP1-06035] - HEK293 overexpressing FANCG and probed with (mock transfection in first lane).

Immunohistochemistry-Paraffin: FANCG Antibody [NBP1-06035] - staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Po, Ca
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

FANCG Antibody Summary

• Immunogen: Peptide with sequence LEEFRTSLPKSCDL corresponding to C-Terminus according to NP_004620.1.
• Epitope: LEEFRTSLPKSCDL
• Predicted Species: Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FANCG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 1 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in HeLa lysates (calculated MW of 68.6 kDa band according to NP_004620.1). In transfected HEK293 transiently expressing FANCG a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293.
  IHC: Paraffin embedded Human Uterus and Spleen.
• Control:

  FANCG Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FANCG Antibody

• DNA repair protein XRCC9
• FAG
• FANCG
• Fanconi anemia group G protein
• Fanconi anemia, complementation group G
• Protein FACG
• X-ray repair, complementing defective, in Chinese hamster, 9
• XRCC9
• XRCC9X-ray repair complementing defective repair in Chinese hamster cells 9

Background

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FANCG Antibody (NBP1-06035)(2)

Publications using NBP1-06035

• Tomida J, Itaya A, Shigechi T et al. A novel interplay between the Fanconi anemia core complex and ATR-ATRIP kinase during DNA cross-link repair. Nucleic Acids Res 2013-05-30 [PMID: 23723247] (WB, Human)
• Liu N, Lamerdin JE, Tucker JD et al. The human XRCC9 gene corrects chromosomal instability and mutagen sensitivities in CHO UV40 cells. Proc Natl Acad Sci U S A 1997-08-19 [PMID: 9256465]

Bioinformatics

• Gene Symbol: FANCG
• Entrez:
  • 2189(Human)
• Uniprot:
  • NP_004620.1(Human)