FAM3C Antibody - BSA Free

Western Blot: FAM3C Antibody [NBP2-24464] - Total protein from human testis tissue and mouse testis tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-FAM3C in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-24464] - Analysis of tissue section of mouse brain using FAM3C antibody at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles.

Western Blot: FAM3C Antibody [NBP2-24464] - Analysis of FAM3C in human brain lysate (0.5 ug/ml) 1) absence, 2) presence of immunizing peptide, 3) mouse brain lysate (0.5 ug/ml), and 4) in rat brain lysate (0.5 ug/ml) using FAM3C antibody.

Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-24464] - Staining of FAM3C using FAM3C antibody in normal human brain at 5 ug/ml (using control rabbit Ig for figure B).

Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-24464] - Analysis of FAM3C in human skin using FAM3C antibody at 10 ug/ml.

Immunohistochemistry-Paraffin: FAM3C Antibody [NBP2-24464] - Analysis tissue section of mouse brain using FAM3C antibody Lot A1 at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles.

Simple Western: FAM3C Antibody [NBP2-24464] - Simple Western lane view shows a specific band for FAM3C in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Ma-Op, Pm
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FAM3C Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit FAM3C Antibody - BSA Free (NBP2-24464) is a polyclonal antibody validated for use in IHC, WB and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 40-80 of human FAM3C was used as the immunogen for this antibody.
• Predicted Species: Opossum (90%), Bovine (100%), Chicken (100%), Canine (100%), Primate (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FAM3C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western 1:20
  • Western Blot 0.5-3 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:10, apparent MW was 27 kDa.

Reactivity Notes

Pufferfish (88%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FAM3C Antibody - BSA Free

• D6WSU176e
• FAM3C
• family with sequence similarity 3, member C
• GS3876
• ILEI
• ILEIInterleukin-like EMT inducer
• predicted osteoblast protein

Background

FAM3C is a member of the family with sequence similarity 3 (FAM3) family and encodes a secreted protein with a GG domain. A change in expression of this protein has been noted in pancreatic cancer-derived cells. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FAM3C
• Uniprot:
  • Q92520()