FAM29A Antibody

Images

 
Western Blot: FAM29A Antibody [NBP2-16416] - Sample (30 ug of whole cell lysate) A: JurKat 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: FAM29A Antibody [NBP2-16416] - ( IFA using NBP2-16416 at 1: 500; red) co-localizes at the mitotic spindle with Hice1-GFP (HAUS-GFP; green) in U2OS cells. DAPI staining (blue) ...read more
Immunohistochemistry-Paraffin: FAM29A Antibody [NBP2-16416] - HAUS6 protein at on colon carcinoma
Western Blot: FAM29A Antibody [NBP2-16416] - IP-WB assay to show that Hice1 co-immunoprecipitated with other Augmin components HAUS6, HAUS2 and HAUS1 in U2OS cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.64 mg/ml

Order Details

FAM29A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HAUS6. The exact sequence is proprietary.
Localization
Cytoplasm; cytoskeleton; Spindle; Centrosome
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HAUS6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
109 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.64 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FAM29A Antibody

  • Dgt6
  • dim gamma-tubulin homolog
  • FAM29AMGC102696
  • family with sequence similarity 29, member A
  • FLJ20060
  • HAUS augmin-like complex subunit 6
  • HAUS augmin-like complex, subunit 6
  • KIAA1574
  • MGC138798
  • MGC138799

Background

HAUS6 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HAUS6
Uniprot