![Western Blot: HAUS8 Antibody [NBP2-42849] - Detection of cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image). Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10).](http://images.novusbio.com/fullsize2/HAUS8-Antibody-Western-Blot-NBP2-42849-img0005.jpg "Western Blot: HAUS8 Antibody [NBP2-42849] - Detection of cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image). Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P, IP, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
Biological Strategies
| Immunogen | The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: HAUS8 Antibody [NBP2-42849] - Analysis of human osteosarcoma cell line U2OS, using HICE1 antibody at 1:50-1:200 dilution.](http://images.novusbio.com/fullsize2/HAUS8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42849-img0001.jpg "Immunocytochemistry/Immunofluorescence: HAUS8 Antibody [NBP2-42849] - Analysis of human osteosarcoma cell line U2OS, using HICE1 antibody at 1:50-1:200 dilution.")
![Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Analysis of Human lymph tissue, using HICE1 antibody at 1:50 dilution.](http://images.novusbio.com/fullsize2/HAUS8-Antibody-Immunohistochemistry-Paraffin-NBP2-42849-img0006.jpg "Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Analysis of Human lymph tissue, using HICE1 antibody at 1:50 dilution.")
![Western Blot: HAUS8 Antibody [NBP2-42849] - Analysis of A: His-Hice1 (2x) B: His-Hice1 (1x) 7.5% SDS PAGE diluted at 1:500.](http://images.novusbio.com/fullsize2/HAUS8-Antibody-Western-Blot-NBP2-42849-img0004.jpg "Western Blot: HAUS8 Antibody [NBP2-42849] - Analysis of A: His-Hice1 (2x) B: His-Hice1 (1x) 7.5% SDS PAGE diluted at 1:500.")
![Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Analysis of Human lymph tissue, using HICE1 antibody at 1:50 dilution.](http://images.novusbio.com/fullsize2/HAUS8-Antibody-Immunohistochemistry-Paraffin-NBP2-42849-img0003.jpg "Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Analysis of Human lymph tissue, using HICE1 antibody at 1:50 dilution.")
![Immunoprecipitation: HAUS8 Antibody [NBP2-42849] - Analysis of Hice1 co-immunoprecipitated with other Augmin components HAUS6, HAUS2 and HAUS1 in U2OS cells.](http://images.novusbio.com/fullsize2/HAUS8-Antibody-Immunoprecipitation-NBP2-42849-img0007.jpg "Immunoprecipitation: HAUS8 Antibody [NBP2-42849] - Analysis of Hice1 co-immunoprecipitated with other Augmin components HAUS6, HAUS2 and HAUS1 in U2OS cells.")
![Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.](http://images.novusbio.com/fullsize2/PLK1-Antibody-(13E8)-Western-Blot-NB100-74502-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in WiDr colon carcinoma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/PLK1-Antibody-(13E8)-Immunocytochemistry-Immunofluorescence-NB100-74502-img0010.jpg)
![Immunohistochemistry-Paraffin: PLK1 Antibody (13E8) [NB100-74502] - Both normal and cancer biopsies of deparaffinized human Colon tissue.](http://images.novusbio.com/fullsize2/PLK1-Antibody-(13E8)-Immunohistochemistry-Paraffin-NB100-74502-img0011.jpg)
![Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in NIH/3T3 ( Cat # L018V1 ).](http://images.novusbio.com/fullsize2/NEDD1-Antibody-(7D10)-Western-Blot-H00121441-M05-img0002.jpg)
![Immunohistochemistry-Paraffin: NEDD1 Antibody (7D10) [H00121441-M05] - Analysis of monoclonal antibody to NEDD1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 0.75 ug/ml](http://images.novusbio.com/fullsize2/NEDD1-Antibody-(7D10)-Immunohistochemistry-Paraffin-H00121441-M05-img0005.jpg)
![Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in Hela NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize2/NEDD1-Antibody-(7D10)-Western-Blot-H00121441-M05-img0001.jpg)
![Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize2/NDC80-Antibody-(9G3.23)-Western-Blot-NB100-338-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] diluted at 1:500. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/NDC80-Antibody-(9G3.23)-Immunocytochemistry-Immunofluorescence-NB100-338-img0017.jpg)
![Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.](http://images.novusbio.com/fullsize2/NDC80-Antibody-(9G3.23)-Western-Blot-NB100-338-img0007.jpg)
![Western Blot: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize2/DNA-Polymerase-delta--catalytic-subunit-Antibody-Western-Blot-NBP2-33466-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize2/DNA-Polymerase-delta--catalytic-subunit-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33466-img0007.jpg)
![Immunohistochemistry-Paraffin: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Staining in human lymph node and liver tissues using anti-POLD1 antibody. Corresponding POLD1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/DNA-Polymerase-delta--catalytic-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-33466-img0010.jpg)
![Western Blot: Nuf2 Antibody [NBP1-31632] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Nuf2-Antibody-Western-Blot-NBP1-31632-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Nuf2 Antibody [NBP1-31632] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDCA1 protein stained by CDCA1 antibody [312] diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/Nuf2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31632-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Nuf2 Antibody [NBP1-31632] - Paraformaldehyde-fixed U2OS, using CDCA1 antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize2/Nuf2 Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31632-img0002.jpg)
![Western Blot: MCAT Antibody [NBP1-83936] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize2/MCAT Antibody-Western Blot-NBP1-83936-img0004.jpg)
![Immunohistochemistry-Paraffin: MCAT Antibody [NBP1-83936] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize2/MCAT-Antibody-Immunohistochemistry-Paraffin-NBP1-83936-img0002.jpg)
![Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize2/CDC2-CDK1-Antibody-Western-Blot-NBP1-85729-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/CDC2-CDK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85729-img0010.jpg)
![Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues. Corresponding CDK1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/CDC2-CDK1 Antibody-Immunohistochemistry-Paraffin-NBP1-85729-img0015.jpg)
![Western Blot: FAM29A Antibody [NBP2-16416] - Sample (30 ug of whole cell lysate) A: JurKat 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/FAM29A-Antibody-Western-Blot-NBP2-16416-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: FAM29A Antibody [NBP2-16416] - ( IFA using NBP2-16416 at 1: 500; red) co-localizes at the mitotic spindle with Hice1-GFP (HAUS-GFP; green) in U2OS cells. DAPI staining (blue) shows mitotic chromosomes. Arrows indicate spindle poles.](http://images.novusbio.com/fullsize2/FAM29A Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16416-img0003.jpg)
![Immunohistochemistry-Paraffin: FAM29A Antibody [NBP2-16416] - HAUS6 protein at on colon carcinoma](http://images.novusbio.com/fullsize2/FAM29A-Antibody-Immunohistochemistry-Paraffin-NBP2-16416-img0004.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.](http://images.novusbio.com/fullsize2/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/MAD1L1-MAD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32775-img0013.jpg)
![Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NBP1-32775] - Paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize2/MAD1L1-MAD1-Antibody-Immunohistochemistry-Paraffin-NBP1-32775-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Mad Antibody [NBP1-89979] - Staining of human cell line U-251MG shows positivity in nucleus but not nucleoli and mitochondria.](http://images.novusbio.com/fullsize2/Mad-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89979-img0003.jpg)
![Immunohistochemistry-Paraffin: Mad Antibody [NBP1-89979] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize2/Mad-Antibody-Immunohistochemistry-Paraffin-NBP1-89979-img0005.jpg)
![Immunohistochemistry-Paraffin: Mad Antibody [NBP1-89979] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize2/Mad-Antibody-Immunohistochemistry-Paraffin-NBP1-89979-img0002.jpg)
![Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize2/MAD2L1 Antibody-Immunohistochemistry-NB100-563-img0006.jpg)
![Immunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2 on HeLa whole cell lysate using NB100-563. MAD2 was also immunoprecipitated using NB100-564. Immunoprecipitated MAD2 was blotted using NB100-564.](http://images.novusbio.com/fullsize2/MAD2L1-Antibody-Immunoprecipitation-NB100-563-img0003.jpg)
![Western Blot: Aurora A Antibody [NBP1-51843] - WB detection of Aurora A in HeLa whole cell lysate.](http://images.novusbio.com/fullsize2/Aurora-A-Antibody-Western-Blot-NBP1-51843-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - IF Confocal analysis of HeLa cells using Aurora A antibody (NBP1-51843, 1:10). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize2/Aurora-A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-51843-img0008.jpg)
![Immunohistochemistry: Aurora A Antibody [NBP1-51843] - IHC staining of Aurora A in mouse brain.](http://images.novusbio.com/fullsize2/Aurora-A-Antibody-Immunohistochemistry-NBP1-51843-img0003.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")
![Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/images2/NDC80-Antibody-(9G3.23)-Western-Blot-NB100-338-img0016.jpg "Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.")