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HAUS8 Antibody

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Western Blot: HAUS8 Antibody [NBP2-42849] - Detection of cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image). Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10).
Biological Strategies: Western Blot: HAUS8 Antibody [NBP2-42849] - Detection of cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image). ...read more
Immunocytochemistry/ Immunofluorescence: HAUS8 Antibody [NBP2-42849] - Analysis of human osteosarcoma cell line U2OS, using HICE1 antibody at 1:50-1:200 dilution.
Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Analysis of Human lymph tissue, using HICE1 antibody at 1:50 dilution.
Western Blot: HAUS8 Antibody [NBP2-42849] - Analysis of A: His-Hice1 (2x) B: His-Hice1 (1x) 7.5% SDS PAGE diluted at 1:500.
Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Analysis of Human lymph tissue, using HICE1 antibody at 1:50 dilution.
Immunoprecipitation: HAUS8 Antibody [NBP2-42849] - Analysis of Hice1 co-immunoprecipitated with other Augmin components HAUS6, HAUS2 and HAUS1 in U2OS cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

      

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HAUS8 Antibody Summary

Immunogen
The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:50-1:500
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for HAUS8 Antibody

  • DGT4
  • HICE1
  • NY-SAR-48

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: PLK1 Antibody (13E8) [NB100-74502] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with PLK1 mouse monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.
Immunocytochemistry/Immunofluorescence: PLK1 Antibody (13E8) [NB100-74502] - PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) in WiDr colon carcinoma cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Western Blot: NEDD1 Antibody (7D10) [H00121441-M05] - Western Blot analysis of NEDD1 expression in NIH/3T3 ( Cat # L018V1 ).
Immunohistochemistry-Paraffin: NEDD1 Antibody (7D10) [H00121441-M05] - Analysis of monoclonal antibody to NEDD1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 0.75 ug/ml
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Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] diluted at 1:500. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.
Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - WB analysis of NDC80 in U2OS Whole Cell Lysate. Verified customer review from 1DegreeBio.
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Immunohistochemistry-Paraffin: Mxi1 Antibody [NBP1-88626] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
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Applications: WB, IHC, IHC-P
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Western Blot: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
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Immunohistochemistry-Paraffin: DNA Polymerase delta, catalytic subunit Antibody [NBP2-33466] - Staining in human lymph node and liver tissues using anti-POLD1 antibody. Corresponding POLD1 RNA-seq data are presented for the same tissues.
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NBP1-31632
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NBP2-42849
HAUS8 Antibody
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Applications: WB, ICC/IF, IHC, IHC-P, IP, KD
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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