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HAUS8 Antibody

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Western Blot: HAUS8 Antibody [NBP2-42849] - Detection of cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image). Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10).
Biological Strategies: Western Blot: HAUS8 Antibody [NBP2-42849] - Detection of cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image). ...read more
Immunocytochemistry/ Immunofluorescence: HAUS8 Antibody [NBP2-42849] - Analysis of human osteosarcoma cell line U2OS, using HICE1 antibody at 1:50-1:200 dilution.
Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Human lymph tissue, using HICE1 antibody at 1:50 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: HAUS8 Antibody [NBP2-42849] - Analysis of A: His-Hice1 (2x) B: His-Hice1 (1x) 7.5% SDS PAGE diluted at 1:500.
Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Human lymph tissue, using HICE1 antibody at 1:50 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunoprecipitation: HAUS8 Antibody [NBP2-42849] - Analysis of Hice1 co-immunoprecipitated with other Augmin components HAUS6, HAUS2 and HAUS1 in U2OS cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

      

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HAUS8 Antibody Summary

Immunogen
Recombinant protein corresponding to human HICE1
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HAUS8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:50-1:500
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated
  • Western Blot 1:500-1:3000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for HAUS8 Antibody

  • DGT4
  • HAUS Augmin Like Complex Subunit 8
  • HEC1/NDC80 interacting, centrosome associated 1
  • HEC1/NDC80-Interacting Centrosome-Associated Protein 1
  • Hec1-Interacting And Centrosome-Associated 1
  • HICE1
  • NY-SAR-48
  • Sarcoma Antigen NY-SAR-48

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol HAUS8