![Western Blot: HAUS8 Antibody [NBP2-42849] - Detection of cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image). Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10).](http://images.novusbio.com/fullsize/HAUS8-Antibody-Western-Blot-NBP2-42849-img0005.jpg "Western Blot: HAUS8 Antibody [NBP2-42849] - Detection of cellular Hice1 and Hice1-GFP expressed in human osteosarcoma U2OS cells (left image), and Hice1 upon siRNA treatment (right image). Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit HAUS8 Antibody - BSA Free (NBP2-42849) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-HAUS8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Recombinant protein corresponding to human HAUS8 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | HAUS8 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
| Publication using NBP2-42849 | Applications | Species |
|---|---|---|
| Stimac V, Koprivec I, Manenica M et al. Augmin prevents merotelic attachments by promoting proper arrangement of bridging and kinetochore fibers eLife 2022-10-21 [PMID: 36269126] (WB, Human) Details: Dilution used in WB 1:1000 |
WB | Human |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | HAUS8 |
![Immunocytochemistry/Immunofluorescence: HAUS8 Antibody [NBP2-42849] - Analysis of human osteosarcoma cell line U2OS, using HICE1 antibody at 1:50-1:200 dilution.](http://images.novusbio.com/fullsize/HAUS8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42849-img0001.jpg "Immunocytochemistry/Immunofluorescence: HAUS8 Antibody [NBP2-42849] - Analysis of human osteosarcoma cell line U2OS, using HICE1 antibody at 1:50-1:200 dilution.")
![Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Human lymph tissue, using HICE1 antibody at 1:50 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HAUS8-Antibody-Immunohistochemistry-Paraffin-NBP2-42849-img0006.jpg "Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Human lymph tissue, using HICE1 antibody at 1:50 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: HAUS8 Antibody [NBP2-42849] - Analysis of A: His-Hice1 (2x) B: His-Hice1 (1x) 7.5% SDS PAGE diluted at 1:500.](http://images.novusbio.com/fullsize/HAUS8-Antibody-Western-Blot-NBP2-42849-img0004.jpg "Western Blot: HAUS8 Antibody [NBP2-42849] - Analysis of A: His-Hice1 (2x) B: His-Hice1 (1x) 7.5% SDS PAGE diluted at 1:500.")
![Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Human lymph tissue, using HICE1 antibody at 1:50 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HAUS8-Antibody-Immunohistochemistry-Paraffin-NBP2-42849-img0003.jpg "Immunohistochemistry-Paraffin: HAUS8 Antibody [NBP2-42849] - Human lymph tissue, using HICE1 antibody at 1:50 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunoprecipitation: HAUS8 Antibody [NBP2-42849] - Analysis of Hice1 co-immunoprecipitated with other Augmin components HAUS6, HAUS2 and HAUS1 in U2OS cells.](http://images.novusbio.com/fullsize/HAUS8-Antibody-Immunoprecipitation-NBP2-42849-img0007.jpg "Immunoprecipitation: HAUS8 Antibody [NBP2-42849] - Analysis of Hice1 co-immunoprecipitated with other Augmin components HAUS6, HAUS2 and HAUS1 in U2OS cells.")
![Immunocytochemistry Mxi1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Mxi1_AF4185_Immunocytochemistry__Immunofluorescence_20286.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")