FADD Antibody

Western blot shows lysates of A431 human epithelial carcinoma cell line, Ramos and Raji human Burkitt's lymphoma cell lines, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human FADD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FADD at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

FADD Antibody Summary

• Immunogen: E. coli-derived recombinant human FADD
  Met1-Ser208
  Accession # Q13158
• Specificity: Detects endogenous human FADD in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FADD
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FADD Antibody

• FADD
• Fas (TNFRSF6)-associated via death domain
• FAS-associated death domain protein
• FAS-associating death domain-containing protein
• GIG3
• Growth-inhibiting gene 3 protein
• Mediator of receptor induced toxicity
• mediator of receptor-induced toxicity
• MGC8528
• MORT1
• MORT1Fas-associating protein with death domain
• protein FADD

Background

Fas-Associating protein with Death Domain (FADD), also known as MORT1, is a 25 kDa adaptor protein that mediates signaling of death domain containing TNF receptor superfamily members. FADD interacts with the death domain of FAS (CD95) and TNFR-1 and recruits procaspase-8 to the Fas signaling complex, initiating caspase activation and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FADD Antibody (AF2938)(1)

Publication using AF2938

• WR English, H Ireland-Ze, AH Baker, TD Littlewood, MR Bennett, G Murphy Tissue Inhibitor of Metalloproteinase-3 (TIMP-3) induces FAS dependent apoptosis in human vascular smooth muscle cells PLoS ONE, 2018-04-04;13(4):e0195116. 2018-04-04 [PMID: 29617412] (Western Blot, Human)

Bioinformatics

• Gene Symbol: FADD
• Uniprot:
  • Q13158()