FADD Antibody (J1D2) - BSA Free

Western Blot: FADD Antibody (J1D2) [NBP1-04288] - Cell lysates of Jurkat (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FADD (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry-Paraffin: FADD Antibody (J1D2) [NBP1-04288] - Paraffin embedded sections of human breast cancer tissue were incubated with anti-human FADD (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: J1D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

FADD Antibody (J1D2) - BSA Free Summary

• Immunogen: Recombinant human FADD (1-208aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FADD
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for FADD Antibody (J1D2) - BSA Free

• FADD
• Fas (TNFRSF6)-associated via death domain
• FAS-associated death domain protein
• FAS-associating death domain-containing protein
• GIG3
• Growth-inhibiting gene 3 protein
• Mediator of receptor induced toxicity
• mediator of receptor-induced toxicity
• MGC8528
• MORT1
• MORT1Fas-associating protein with death domain
• protein FADD

Background

FADD (Fas-associated protein with death domain) is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. This protein is implicated in survival/proliferation and cell cycle progression. FADD functions are also regulated via cellular sublocalization, protein phosphorylation, and inhibitory molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FADD
• Uniprot:
  • NP_003815(Human)