Rat Factor XIII ELISA Kit (Colorimetric)

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ELISA: Rat Factor XIII ELISA Kit (Colorimetric) [NBP2-82475] - Samples were spiked with high concentrations of Rat Factor XIII and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Rat Factor XIII ELISA Kit (Colorimetric) [NBP2-82475] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL

Order Details

Rat Factor XIII ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Factor XIII in samples. No Significant cross-reactivity or interference between Rat Factor XIII and analogues was observed.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.55
Intra-Assay
CV% < 4.96
Spike Recovery
87-105%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
F13B

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Factor XIII ELISA Kit (Colorimetric)

  • bA525O21.1 (coagulation factor XIII, A1 polypeptide)
  • coagulation factor XIII A chain
  • coagulation factor XIII, A1 polypeptide
  • Coagulation factor XIIIa
  • EC 2.3.2.13
  • F13Acoagulation factor XIII, A polypeptide
  • factor XIIIa
  • fibrin stabilizing factor, A subunit
  • fibrinoligase
  • FSF, A subunit
  • Protein-glutamine gamma-glutamyltransferase A chain
  • TGase
  • Transglutaminase A chain
  • transglutaminase. plasma

Background

Factor XIII is activated by thrombin and calcium ions to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. It also cross-links alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII belongs to the transglutaminase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol F13B