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Human Factor XIII ELISA Kit (Colorimetric)

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ELISA: Human Factor XIII ELISA Kit (Colorimetric) [NBP2-60505]
ELISA: Human Factor XIII ELISA Kit (Colorimetric) [NBP2-60505]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell Culture, Urine, Saliva, Milk, CSF
Standard Curve Range
2.5-160 ng/ml (example only; lot dependent)
Sensitivity
2.1 ng/ml (example only; lot dependent)

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Human Factor XIII ELISA Kit (Colorimetric) Summary

Description
This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
Standard Curve Range
2.5-160 ng/ml (example only; lot dependent)
Sensitivity
2.1 ng/ml (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 9.40% (example only; lot dependent)
Intra-Assay
CV% < 4.40% (example only; lot dependent)
Spike Recovery
98.00% (example only; lot dependent)
Kit Type
ELISA Kit (Colorimetric)
Gene
F13B

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage is content dependent.

Alternate Names for Human Factor XIII ELISA Kit (Colorimetric)

  • bA525O21.1 (coagulation factor XIII, A1 polypeptide)
  • coagulation factor XIII A chain
  • coagulation factor XIII, A1 polypeptide
  • Coagulation factor XIIIa
  • EC 2.3.2.13
  • F13Acoagulation factor XIII, A polypeptide
  • factor XIIIa
  • fibrin stabilizing factor, A subunit
  • fibrinoligase
  • FSF, A subunit
  • Protein-glutamine gamma-glutamyltransferase A chain
  • TGase
  • Transglutaminase A chain
  • transglutaminase. plasma

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP1-86951] - Staining of human endometrium, fallopian tube, placenta and testis using Anti-TGM2 antibody NBP1-86951 (A) shows similar protein distribution across tissues to independent antibody NBP1-86952 (B).
Western Blot: Transglutaminase 2/TGM2 Antibody [NBP1-86951] - Analysis using Anti-TGM2 antibody NBP1-86951 (A) shows similar pattern to independent antibody NBP1-86952 (B).
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Factor XIIIa
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NBP2-80399
Flow Cytometry: Factor XIIIa Antibody (16D1) [NBP2-80399] - Analysis of Factor XIIIa was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).
Immunocytochemistry/Immunofluorescence: Factor XIIIa Antibody (16D1) [NBP2-80399] - Staining of F13A1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-39, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Immunocytochemistry/Immunofluorescence: Factor XIIIa Antibody (16D1) [NBP2-80399] - Staining of F13A1 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-39, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Lipoprotein Lipase/LPL was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human Lipoprotein Lipase/LPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7197) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog&amp;nbsp;# NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. This application has not been tested in mouse samples.
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Plasminogen
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NB600-930
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Human Factor XIII
ELISA Kit (Colorimetr ...
NBP2-60505
Human Factor XIII ELISA Kit (Colorimetric)
Species: Hu
Applications: ELISA

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Bioinformatics

Gene Symbol F13B