FACL4 Antibody (RB04697)

Product Discontinued

FACL4 Antibody (RB04697) Summary

• Immunogen: This ACSL4 (FACL4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-267 amino acids from the Central region of human ACSL4 (FACL4).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACSL4
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • Western Blot 1:1000
• Theoretical MW: 79.188 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 6.3 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for FACL4 Antibody (RB04697)

• ACS4mental retardation, X-linked 68
• acyl-CoA synthetase 4
• acyl-CoA synthetase long-chain family member 4
• EC 6.2.1.3
• FACL4long-chain 4
• LACS 4
• LACS4MRX68
• lignoceroyl-CoA synthase
• Long-chain acyl-CoA synthetase 4
• long-chain fatty-acid-Coenzyme A ligase 4
• long-chain-fatty-acid--CoA ligase 4
• mental retardation, X-linked 63
• MRX63

Background

Long chain acyl-CoA synthetase (LACS), or long chain fatty acid-CoA ligase (FACL), converts free long chain fatty acids into fatty acyl-CoA esters, key intermediates in the synthesis of complex lipids. The FACL4 gene encodes a form of LACS and is expressed in several tissues, including brain. FACL4 cDNA from brain encodes a gene product that shows preference for arachidonic acid as a substrate when expressed in mammalian cells.1 The sequence of the predicted 670-amino acid human protein is 97% identical to that of rat ACS4. FACL4 is highly expressed in adult human brain, especially in the cerebellum and hippocampus, similar to the mouse.2 A strong cytoplasmic staining was found in the Purkinje and granular cells of the cerebellum and the pyramidal layer of hippocampus, indicating that FACL4 is specifically expressed in neurons and not in glial cells. Two patients with Alport syndrome, elliptocytosis, and mental retardation carried a large deletion of the COL4A5 region that included FACL4.3 The absence of FACL4 might play a role in the development of mental retardation or other signs associated with Alport syndrome. Two point mutations, 1 missense and 1 splice site change, were reported in the FACL4 gene in 2 families with nonspecific mental retardation.2 Analysis of enzymatic activity in lymphoblastoid cell lines of affected individuals revealed low levels compared with normal cells, indicating that both mutations are null mutations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACSL4
• Uniprot:
  • O60488(Human)