FACA/FANCA Antibody

Western Blot: FACA/FANCA Antibody [NBP1-03286] - Anti-FANCA Antibody - Western Blot. Western blot using affinity purified anti-FANCA antibody shows detection of FANCA only in FANCA transfected GM6914 cell lysates. No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection. Modified from Smogorzewska et al (2007) Cell 129, 289-301.

Immunohistochemistry-Paraffin: FACA/FANCA Antibody [NBP1-03286] - Staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western Blot: FACA/FANCA Antibody [NBP1-03286] - Anti-FANCA Antibody - Western Blot. Western blot using affinity purified anti-FANCA antibody shows detection of a band at ~133 kD (arrowhead) corres-ponding to FANCA in HeLa whole cell lysates. The identity of the lower molecular weight bands is unknown but may represent breakdown products.

Product Discontinued

FACA/FANCA Antibody Summary

• Immunogen: Synthetic peptide from human FACA/FANCA corresponding to aa 995-1009.
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FANCA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:15000 - 1:60000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Immunoprecipitation
  • Western Blot 1:500 - 1:3000
• Application Notes: ICC/IF and IP usage were reported in scientific literature (PMID: 24469828).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for FACA/FANCA Antibody

• FA
• FA1
• FAA
• FAAMGC75158
• FACA
• FACAFanconi anemia group A protein
• FAH
• FA-H
• FANCA
• FANCH
• Fanconi anemia, complementation group A
• Fanconi anemia, complementation group H
• Fanconi anemia, type 1
• Protein FACA

Background

FANCA (also called Protein FACA or Fanconi anemia group A protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCA may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, and FANCL. The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group A. Alternative splicing results in multiple transcript variants encoding different isoforms. Variant 1 (isoform a) encodes the longest transcript. Variant 2 (isoform b) contains an alternate exon, which results in an early stop codon, compared to variant 1. Isoform b has a shorter C-terminus when compared to isoform a. Mutations in this gene are the most common cause of Fanconi anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FACA/FANCA Antibody (NBP1-03286)(1)

Publication using NBP1-03286

• Huard CC, Tremblay CS, Magron A, Carreau M. The Fanconi anemia pathway has a dual function in Dickkopf-1 transcriptional repression. Proc. Natl. Acad. Sci. U.S.A. 2014-02-12 [PMID: 24469828] (WB, ICC/IF, IP, Human)

Bioinformatics

• Gene Symbol: FANCA
• Entrez:
  • 2175(Human)
• Uniprot:
  • O15360(Human)