Ezrin Antibody (CL2360) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG

Staining of A-431 cells using the Anti-Ezrin monoclonal antibody) .

Analysis in human placenta and skeletal muscle tissues using NBP3-44476 antibody. Corresponding Ezrin RNA-seq data are presented for the same tissues.

Staining of human placenta shows strong membranous positivity in trophoblastic cells.

Staining of human rectum shows strong membranous positivity in glandular cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human tonsil shows strong membranous positivity in non-germinal center cells.

Staining of U-251 cells using the Anti-Ezrin monoclonal antibody) .

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL2360
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Ezrin Antibody (CL2360) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Ezrin Antibody (CL2360) - Azide and BSA Free (NBP2-36761) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P15311, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: EZR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Ezrin Antibody (CL2360) - Azide and BSA Free

• CVIL
• CVL
• Cytovillin 2
• Cytovillin
• DKFZp762H157
• EZR
• Ezrin
• FLJ26216
• p81
• VIL2
• VIL2MGC1584
• villin 2 (ezrin)
• Villin 2
• villin-2

Background

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EZR