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Ezrin Antibody (2086C) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow
Clone
2086C
Clonality
Monoclonal
Host
Rabbit
Conjugate
Alexa Fluor 594

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC72391U-100UG
Alexa Fluor 405
IC72391V-100UG
Alexa Fluor 488
IC72391G-100UG
Alexa Fluor 647
IC72391R-100UG
Alexa Fluor 700
IC72391N-100UG
Alexa Fluor 750
IC72391S-100UG
Unconjugated
MAB72391-100
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB72391-100
Carrier Free
MAB72391-100
Carrier Free
MAB72391-SP
Carrier Free
IC72391U-100UG
Carrier Free
IC72391S-100UG
Carrier Free
IC72391R-100UG
Carrier Free
IC72391N-100UG
Carrier Free
IC72391G-100UG
Carrier Free
IC72391V-100UG
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Ezrin Antibody (2086C) [Alexa Fluor® 594] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E. coli-derived recombinant human Ezrin
Lys438-Arg562
Accession # P15311
Specificity
Detects human Ezrin in direct ELISAs and detects human, mouse, and rat Ezrin in Western blots.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ezrin Antibody (2086C) [Alexa Fluor® 594]

  • CVIL
  • CVL
  • Cytovillin 2
  • Cytovillin
  • DKFZp762H157
  • EZR
  • Ezrin
  • FLJ26216
  • p81
  • VIL2
  • VIL2MGC1584
  • villin 2 (ezrin)
  • Villin 2
  • villin-2

Background

EZRIN (also Cytovillin, Villin2 and p81) is a founding member of the ERM family, Band 4.1 Superfamily of proteins. Although its predicted MW is 69 kDa, it runs anomalously at 77-82 kDa in SDS-PAGE. ERZIN is expressed by epithelial cells where it serves as a linker between the cell membrane and the actin cytoskeleton. Its presence is particularly strong in microvilli where it helps organize this structure. In addition, ERZIN also organizes microtubules in lymphocytes at or near the immunological synapse by interacting with Glg1. Human EZRIN is 585 amino acids (aa) in length. It contains a band 4.1 homology/FERM domain that binds CD44, ICAM-1, EBP50 and ERM family members (aa 1-295), a central alpha -helical region (aa 296-352), and a C-terminal ERM and actin-binding/FERM C domain (aa 353-586). EZRIN exists as either a monomer, or a homo/heterodimer. EZRIN is not constitutively active, but must be phosphorylated and unfolded to bind to cytoplasmic proteins. Over aa 438-562, human EZRIN shares 96% aa identity with mouse EZRIN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Radixin Antibody (SC06-22) [NBP2-67584] - Staining Radixin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Western Blot: NF2/Merlin Antibody [NBP1-87757] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
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Western Blot: Rhodopsin Antibody (1D4) [NBP1-47602] - Analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
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RhoD Antibody
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CD44 Antibody
(8E2F3)
NBP1-47386
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RhoA Antibody
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AKT [p Ser473]
Antibody - Pan Specif
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HGF [Unconjugated]
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Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is 0.1‑0.5 ng/mL.
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Tight Junction
Protein 1 Antibody
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Src [p Tyr419]
Antibody [Unconjugate
AF2685
Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Src (Y419) at approximately 56 - 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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(1 Review)
(5 Publications)
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Applications: WB, Simple Western, IHC
CFTR Antibody
(24-1) [Unconjugated] ...
MAB25031
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Mouse Anti-Human CFTR C‑Terminus Monoclonal Antibody (Catalog # MAB25031) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR C-Terminus Monoclonal Antibody (Catalog # MAB25031) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(11 Reviews)
(41 Publications)
Species: Hu
Applications: WB, IHC, IP
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
(33 Reviews)
(647 Publications)
Species: Hu
NHERF-2 Antibody
NB100-2450
Western Blot: NHERF-2 Antibody [NB100-2450] - (0.3ug/ml) Analysis of human kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NHERF-2 Antibody [NB100-2450] - IHC analysis of NHERF-2 in paraffin embedded Human Kidney using NB100-2450 at 3ug/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
Ezrin Antibody
(2086C) [Alexa Fluor& ...
IC72391T
Ezrin Antibody (2086C) [Alexa Fluor® 594]
Species: Hu, Mu, Rt
Applications: Flow

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC72391U
Alexa Fluor 405 IC72391V
Alexa Fluor 488 IC72391G
Alexa Fluor 594 IC72391T
Alexa Fluor 647 IC72391R
Alexa Fluor 700 IC72391N
Alexa Fluor 750 IC72391S
Learn about Custom Antibody Labeling

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