EZH2/KMT6 [p Ser21] Antibody

Immunohistochemistry-Paraffin: EZH2/KMT6 [p Ser21] Antibody [NBP1-18935] - FFPE serial sections of mouse renal cell carcinoma. Mock phosphatase treatedsection (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-PhosphoEZH2 (S21) sed at a dilution of 1:250. Detection: DAB

Immunohistochemistry-Paraffin: EZH2/KMT6 [p Ser21] Antibody [NBP1-18935] - FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit antiPhospho-EZH2 (S21) used at a dilution of 1:250. Detection:DAB

Product Discontinued

EZH2/KMT6 [p Ser21] Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to phosphorylated Serine 21 and surrounding residues of humanKMT6/EZH2 [p Ser21] using the numbering given in entry NP_004447.2 (GeneID 2146).
• Modification: p Ser21
• Predicted Species: Rat (100%), Porcine (100%), Rabbit (100%), Guinea Pig (100%), Bovine (100%), Chicken (100%), Canine (100%), Primate (100%), Turkey (100%), Equine (100%), Monkey (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EZH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
• Application Notes: Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections.

Reactivity Notes

Orangutan (100%), Panda (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for EZH2/KMT6 [p Ser21] Antibody

• EC 2.1.1
• EC 2.1.1.43
• enhancer of zeste (Drosophila) homolog 2
• enhancer of zeste 2
• enhancer of zeste homolog 2 (Drosophila)
• Enhancer of zeste homolog 2
• ENX1
• ENX-1KMT6EZH1KMT6A
• EZH2
• histone-lysine N-methyltransferase EZH2
• KMT6
• Lysine N-methyltransferase 6
• MGC9169

Background

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EZH2/KMT6 Antibody (NBP1-18935)(1)

Publication using NBP1-18935

• HylPL, McDade SS, McCloskey R et al. Evidence for alteration of EZH2, BMI1, and KDM6A and epigenetic reprogramming in human papillomavirus type 16 E6/E7-expressing keratinocytes. J Virol 2011-11-01 [PMID: 21865393]

Bioinformatics

• Gene Symbol: EZH2
• Entrez:
  • 2146(Human)
  • 14056(Mouse)
• Uniprot:
  • NP_004447.2(Human)