EVI2B Antibody - Azide and BSA Free

Western Blot: EVI2B Antibody [NBP2-99056] - Lane A: Mouse heart tissue lysate Whole Cell LysateLane B: Mouse spleen tissue lysate Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:49 kDaObserved band size:49 kDa

Immunoprecipitation: EVI2B Antibody [NBP2-99056] - Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate1 uL anti-EVI2B rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EVI2B rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 49 kDaObserved band size: 49 kDa

• Reactivity: Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

EVI2B Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Mouse EVI2B.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EVI2B
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 0.2-1 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for EVI2B Antibody - Azide and BSA Free

• CD361 antigen
• D17S376
• Ecotropic viral integration site 2B protein homolog
• ecotropic viral integration site 2B
• EVDBprotein EVI2B
• EVI-2B

Background

The EVI2B gene encodes a 448 amino acid long, 48 kDA EVI2B protein that is frequently researched regarding its role in neurofibromatosis, leukemia, and myeloid leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EVI2B