ETV1 Antibody (815207) [Unconjugated]

Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ETV1 Monoclonal Antibody (Catalog # MAB9389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ETV1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 815207
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

ETV1 Antibody (815207) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human ETV1
  Asn120-Leu190
  Accession # P50549
• Specificity: Detects human ETV1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ETV1 Antibody (815207) [Unconjugated]

• DKFZp781L0674
• ER81
• ER81ets variant gene 1
• ETS translocation variant 1
• ets variant 1
• Ets-related protein 81
• ETV1
• MGC104699
• MGC120533
• MGC120534

Background

ETS translocation variant 1 is a protein that in humans is encoded by the ETV1 gene. ETV-1, ETV-4 and ETV-5 proteins form the PEA3 subfamily of ETS transcription factors. Posttranslational modifications in this subfamily promote cell proliferation, motility and invasion.  Disregulation of ETV-1 has been observed in gastrointestinal stromal and protatic tumors, and in triple-negative breast cancer. Human ETV-1 is a 477 aminoacids (aa) protein with at least six known isoforms. Over the immunogen used in this antibody (aa120-190), ETV-1 sequences are 96% identical to mouse and rat ETV-1 sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P50549()