ERR alpha/NR3B1 Antibody - BSA Free

Western Blot: ERR alpha/NR3B1 Antibody [NBP2-16380] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:2000.

Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP2-16380] - Human intestine. ERR alpha stained by ERR alpha antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Chromatin Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.

Immunoprecipitation: ERR alpha/NR3B1 Antibody [NBP2-16380] - IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 ug of ERR alpha antibody 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary eagent.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ERR alpha/NR3B1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ERR alpha/NR3B1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (98%), Rat (98%), Porcine (99%), Bovine (99%), Canine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ESRRA
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) Assay dependent
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin Assay dependent
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ERR alpha/NR3B1 Antibody - BSA Free

• ERR alpha
• ERR1
• ERR1Estrogen receptor-like 1
• ERRa
• ESRL1
• ESRL1ERR-alpha
• ESRRA
• estrogen-related receptor alphaERRalpha
• NR3B1
• NR3B1Nuclear receptor subfamily 3 group B member 1
• steroid hormone receptor ERR1

Background

The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ERR alpha/NR3B1 Antibody (NBP2-16380)(1)

Publication using NBP2-16380

• Bucher M, Montaniel KRC, Myatt L et al. Dyslipidemia, insulin resistance, and impairment of placental metabolism in the offspring of obese mothers Journal of Developmental Origins of Health and Disease 2021-10-01 [PMID: 33185172] (Western Blot)

Bioinformatics

• Gene Symbol: ESRRA
• Uniprot:
  • P11474(Human)