ERp72 Antibody

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Western Blot: ERp72 Antibody [NB120-11422] - HT 1080 cell lysate.

Product Details

Summary
Product Discontinued
View other related ERp72 Primary Antibodies

Order Details


    • Catalog Number
      NB120-11422
    • Availability
      Product Discontinued

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ERp72 Antibody Summary

Immunogen
Synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of humna ERp72.
Localization
Endoplasmic reticulum
Marker
Endoplasmic Reticulum Marker
Specificity
This detects ERp72 protein in human samples.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDIA4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
Application Notes
WB: Detects an approx. 72 kDa protein representing ERp72 in human fibrosarcoma HT 1080 cell lysate.

Reactivity Notes

Expected cross-reactivity with Porcine (94%), Mouse and Rat (88%) base on sequence homology.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ERp72 Antibody

  • Endoplasmic reticulum resident protein 70
  • Endoplasmic reticulum resident protein 72
  • ER protein 70
  • ERp70
  • ERP70ER protein 72
  • ERp72
  • ERp-72
  • ERP72EC 5.3.4.1
  • intestinal-related)
  • protein disulfide isomerase family A, member 4
  • protein disulfide isomerase related protein (calcium-binding protein
  • protein disulfide isomerase-associated 4
  • protein disulfide-isomerase A4

Background

ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PDIA4
Entrez