ERp72 Antibody - BSA Free

Western Blot: ERp72 Antibody [NB120-11422] - HT 1080 cell lysate.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ERp72 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of humna ERp72.
• Localization: Endoplasmic reticulum
• Marker: Endoplasmic Reticulum Marker
• Specificity: This detects ERp72 protein in human samples.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDIA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:500
  • Western Blot 1:100 - 1:1000
• Application Notes: WB: Detects an approx. 72 kDa protein representing ERp72 in human fibrosarcoma HT 1080 cell lysate.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse and Rat (88%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ERp72 Antibody - BSA Free

• Endoplasmic reticulum resident protein 70
• Endoplasmic reticulum resident protein 72
• ER protein 70
• ERp70
• ERP70ER protein 72
• ERp72
• ERp-72
• ERP72EC 5.3.4.1
• intestinal-related)
• protein disulfide isomerase family A, member 4
• protein disulfide isomerase related protein (calcium-binding protein
• protein disulfide isomerase-associated 4
• protein disulfide-isomerase A4

Background

ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDIA4
• Entrez:
  • 9601(Human)