ERp72 Antibody (10-F2)

Western Blot: ERp72 Antibody (10-F2) [NBP2-76846] - Western blot analysis of ERp72 on A431 cell lysate using anti-ERp72 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: ERp72 Antibody (10-F2) [NBP2-76846] - ICC staining ERp72 (green) in SKOV-3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ERp72 Antibody (10-F2) [NBP2-76846] - Analysis of mouse brain tissue using anti-ERp72 antibody. Counter stained with hematoxylin.

Flow Cytometry: ERp72 Antibody (10-F2) [NBP2-76846] - Flow cytometric analysis of SiHa cells with ERp72 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: ERp72 Antibody (10-F2) [NBP2-76846] - ICC staining ERp72 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ERp72 Antibody (10-F2) [NBP2-76846] - Analysis of rat testis tissue using anti-ERp72 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ERp72 Antibody (10-F2) [NBP2-76846] - Analysis of human lung cancer tissue using anti-ERp72 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ERp72 Antibody (10-F2) [NBP2-76846] - Analysis of human liver tissue using anti-ERp72 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ERp72 Antibody (10-F2) [NBP2-76846] - Analysis of human placenta tissue using anti-ERp72 antibody. Counter stained with hematoxylin.

Product Discontinued

ERp72 Antibody (10-F2) Summary

• Immunogen: Synthetic peptide within Human ERp72 aa 596-645 / 645. (SwissProt: P13667 Human; SwissProt: P08003 Mouse; SwissProt: P38659 Rat)
• Localization: Endoplasmic reticulum.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDIA4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for ERp72 Antibody (10-F2)

• Endoplasmic reticulum resident protein 70
• Endoplasmic reticulum resident protein 72
• ER protein 70
• ERp70
• ERP70ER protein 72
• ERp72
• ERp-72
• ERP72EC 5.3.4.1
• intestinal-related)
• protein disulfide isomerase family A, member 4
• protein disulfide isomerase related protein (calcium-binding protein
• protein disulfide isomerase-associated 4
• protein disulfide-isomerase A4

Background

ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDIA4