ERp57/PDIA3 Antibody - BSA Free

Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody [C3], C-term diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NB100-78614] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERp57 protein stained by ERp57 antibody [C3], C-term diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Paraffin-embedded mouse lymph node. ERp57 antibody [C3], C-term dilution: 1:500.

Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7.5% SDS PAGE diluted at 1:1000

Western Blot: ERp57/PDIA3 Antibody [NB100-78614] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:10000

Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NB100-78614] - Sample: MCF-7 cells were fixed in iced-cold MeOH for 5 min. Green: PDIA3 protein stained by ERp57 antibody, C-term diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - H1299 xenograft, using ERp57 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Mouse intestine. ERp57 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NB100-78614] - Rat middle brain. ERp57 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm, RM, Sh
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ERp57/PDIA3 Antibody - BSA Free Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ERp57/PDIA3. The exact sequence is proprietary.
• Localization: Cell membrane
• Marker: Endoplasmic Reticulum Marker
• Predicted Species: Porcine (100%), Chimpanzee (100%), Bovine (100%), Rhesus Macaque (100%), Sheep (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDIA3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  ERp57/PDIA3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Protein A purified

Alternate Names for ERp57/PDIA3 Antibody - BSA Free

• Disulfide isomerase ER-60
• EC 5.3.4.1
• endoplasmic reticulum P58
• Endoplasmic reticulum resident protein 57
• Endoplasmic reticulum resident protein 60
• ER protein 60
• ER60
• ERp57
• ERp5758 kDa glucose-regulated protein
• ERp60
• ERp6058 kDa microsomal protein
• ERp61
• glucose regulated protein, 58kDa
• GRP57
• GRP58
• GRP58ER protein 57
• HsT17083
• P58
• PDIA3
• phospholipase C-alpha
• PI-PLC
• protein disulfide isomerase family A, member 3
• protein disulfide isomerase-associated 3
• protein disulfide-isomerase A3

Background

Protein Disulfide Isomerase A3(PDIA3) was also names as GRP58, which was first identified by Bourdi et al. (1995), Koivunen et al. (1996), and Hirano et al. (1995). PDIA3 was coded by 505 aa with disulfide isomerase homology. PIDA3 was localizated in nuclear and ER with specific sequence. PDIA3 was mojorily expressed in liver, placenta, and lung, and lower expressed in all other tissues tested. PDIA4 had protein disulfide isomerase activity, thiol-dependent reductase activity, and induced by oncogenic transformation (Koivunen et al., 1996; Hirano et al., 1995)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PDIA3
• Entrez:
  • 2923(Human)