ERK5/BMK1 Antibody [DyLight 650]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: DyLight 650

ERK5/BMK1 Antibody [DyLight 650] Summary

• Immunogen: E. coli-derived recombinant human ERK5/BMK1
  Met1-Glu432
  Accession # Q13164
• Specificity: Detects human and mouse ERK5/BMK1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MAPK7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for ERK5/BMK1 Antibody [DyLight 650]

• Big MAP kinase 1
• BMK1 kinase
• BMK1
• BMK-1
• BMK1EC 2.7.11.24
• EC 2.7.11
• ERK5
• ERK-5
• ERK5MAPK 7
• Extracellular signal-regulated kinase 5
• extracellular-signal-regulated kinase 5
• MAP kinase 7
• MAPK7
• mitogen-activated protein kinase 7
• PRKM7ERK4

Background

The extracellular signal-regulated kinase 5 (ERK5), also known as MAPK7 or big mitogen-activated protein kinase 1 (BMK1), is a member of the MAP kinase subfamily (1). ERK5 differs considerably from other MAPKs; it contains an unusually long carboxyl-terminal tail which might contribute to the regulation of its activity and/or localization. In response to extracellular signals, ERK5 translocates to the nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors (2). ERK5 also differs from other MAPKs in possessing a potent transcriptional activation domain which mediates protein-protein interactions with the myocyte enhancer factor 2 (MEF2) transcription factors (3). ERK5 is specifically activated by MAPK kinase 5 (MAP2K5/MEK5) and plays an important role in mammary epithelial proliferation, endothelial cell survival and normal embryonic development (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK7