ERK5/BMK1 Antibody [mFluor Violet 500 SE]

• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: mFluor Violet 500 SE

ERK5/BMK1 Antibody [mFluor Violet 500 SE] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: E. coli-derived recombinant human ERK5/BMK1
  Met1-Glu432
  Accession # Q13164
• Specificity: Detects human and mouse ERK5/BMK1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MAPK7
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product AF2848. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ERK5/BMK1 Antibody [mFluor Violet 500 SE]

• Big MAP kinase 1
• BMK1 kinase
• BMK1
• BMK-1
• BMK1EC 2.7.11.24
• EC 2.7.11
• ERK5
• ERK-5
• ERK5MAPK 7
• Extracellular signal-regulated kinase 5
• extracellular-signal-regulated kinase 5
• MAP kinase 7
• MAPK7
• mitogen-activated protein kinase 7
• PRKM7ERK4

Background

The extracellular signal-regulated kinase 5 (ERK5), also known as MAPK7 or big mitogen-activated protein kinase 1 (BMK1), is a member of the MAP kinase subfamily (1). ERK5 differs considerably from other MAPKs; it contains an unusually long carboxyl-terminal tail which might contribute to the regulation of its activity and/or localization. In response to extracellular signals, ERK5 translocates to the nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors (2). ERK5 also differs from other MAPKs in possessing a potent transcriptional activation domain which mediates protein-protein interactions with the myocyte enhancer factor 2 (MEF2) transcription factors (3). ERK5 is specifically activated by MAPK kinase 5 (MAP2K5/MEK5) and plays an important role in mammary epithelial proliferation, endothelial cell survival and normal embryonic development (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK7