ERK2 Antibody


Western Blot: ERK2 Antibody [NBP1-19102] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50ug) cells. Antibody used at for WB at 0.1 ug/ml.
Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-19102] - Human breast carcinoma. Antibody used at a dilution of 1:1,000.
Immunoprecipitation: ERK2 Antibody [NBP1-19102] - Samples: 1 mg whole cell lysate was Immunoprecipitated from HeLa cells that were mock treated (-) or treated with TPA (+); 20% of the IP was loaded. Antibodies: In (A), more
Proximity Ligation Assay: ERK2 Antibody [NBP1-19102] - Image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), more

Product Details

Reactivity Hu, Mu, Bovine, Canine, Chicken, Equine, Guinea Pig, Primate, Porcine, Rabbit, Rat, Turkey, MonkeySpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PLA, IP (-)
1.0 mg/ml

Order Details

ERK2 Antibody Summary

The immunogen recognized by this antibody maps to a region between residue 310 and 360 of human extracellular signal-regulated kinase-2 (mitogen-activated protein kinase 1) using the numbering given in entry NP_002736.3 (GeneID 5594).
Predicted Species
Rat (100%), Porcine (100%), Guinea Pig (100%), Bovine (100%), Rabbit (100%), Chicken (100%), Canine (100%), Equine (100%), Primate (100%), Turkey (100%), Monkey (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


  • Immunoprecipitation (Negative)
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:250 - 1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Proximity Ligation Assay 1:1000-1:20000
Application Notes
Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee,Tasmanian devil, White-tufted-ear marmoset, Crab-eating macaque and Northern white-cheeked gibbon.


This anitbody can be used as the primaryantibody in a PLA assay with the following as secondary antibodies: NB100-1567, NB100-312, NB100-314

Alternate Names for ERK2 Antibody

  • EC 2.7.11
  • EC
  • ERK
  • ERK-2
  • ERK2MAP kinase isoform p42
  • ERT1
  • Extracellular signal-regulated kinase 2
  • MAP kinase 1
  • MAP kinase 2
  • MAPK 1
  • MAPK1
  • MAPK2
  • mitogen-activated protein kinase 1
  • Mitogen-activated protein kinase 2
  • p38
  • p40
  • p41
  • p41mapk
  • p42mapk
  • p42-MAPK
  • PRKM1
  • PRKM2
  • protein tyrosine kinase ERK2


The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol MAPK1

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