ERK1/ERK2 [p Thr185, p Tyr187] Antibody

Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ERK1 (phospho Thr202/Tyr204) / ERK2 (phospho Thr185/Tyr187) antibody (NBP3-13137) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) / ERK2 (phospho Thr185/Tyr187) antibody (NBP3-13137) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: ERK1/ERK2 [p Thr185, p Tyr187] Antibody [NBP3-13137] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1 (phospho Thr202/Tyr204) / ERK2 (phospho Thr185/Tyr187) antibody (NBP3-13137) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ERK1/ERK2 [p Thr185, p Tyr187] Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Thr202/Tyr204 of human ERK1/ERK2. The exact sequence is proprietary.
• Modification: p Thr185, p Tyr187
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ERK1/ERK2 [p Thr185, p Tyr187] Antibody

• ERK1
• ERK-1
• ERK1/ERK2
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAPK 1
• Microtubule-associated protein 2 kinase
• mitogen-activated protein kinase 3
• p44erk1
• p44-ERK1
• p44mapk
• p44-MAPK
• PRKM3

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK3