ERK1/ERK2 Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line myoblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat ERK1/ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1576) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879‑KS) and Recombinant Human Active ERK2 (Catalog # 1230‑KS) (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for ERK1/ERK2 at approximately 44 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK1/ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1576). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

ERK1/ERK2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human ERK1/ERK2
  XM_055766 and NM_138957, respectively
• Specificity: Detects human, mouse and rat ERK1/ERK2.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL
  • Simple Western 10 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ERK1/ERK2 Antibody [Unconjugated]

• ERK1/ERK2

Background

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44 and 42 kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2.

ERK5, also known as Big Mitogen-activated Protein Kinase 1 (BMK1) and MAPK7, is activated by several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERK1/ERK2 Antibody (AF1576)(11)

Publications using AF1576

• P Barroso-Ch, D Luis-Ravel, F Fumagallo-, J Castro-Her, J Salas-Hern, J Rodriguez-, A Febles-Cas, I Cruz-Muros, D Afonso-Ora, P Abreu-Gonz, R Moratalla, MJ Millan, T Gonzalez-H DRD3 (dopamine receptor D3) but not DRD2 activates autophagy through MTORC1 inhibition preserving protein synthesis Autophagy, 2019;0(0):. 2019 [PMID: 31538542] (Western Blot, Human)
• SM Herbrich, S Kannan, RM Nolo, M Hornbaker, J Chandra, PA Zweidler-M Characterization of TRKA signaling in acute myeloid leukemia Oncotarget, 2018;9(53):30092-30105. 2018 [PMID: 30046390] (Western Blot, Human)
• MC Joo, CH Jang, JT Park, SW Choi, S Ro, MS Kim, MY Lee Effect of electrical stimulation on neural regeneration via the p38-RhoA and ERK1/2-Bcl-2 pathways in spinal cord-injured rats Neural Regen Res, 2018;13(2):340-346. 2018 [PMID: 29557386] (Western Blot, Rat)
• H Ungefroren, D Witte, C Fiedler, T Gädeken, R Kaufmann, H Lehnert, F Gieseler, BH Rauch The Role of PAR2 in TGF-?1-Induced ERK Activation and Cell Motility Int J Mol Sci, 2017;18(12):. 2017 [PMID: 29261154] (Western Blot, Human)
• D Witte, H Otterbein, M Förster, K Giehl, R Zeiser, H Lehnert, H Ungefroren Negative regulation of TGF-?1-induced MKK6-p38 and MEK-ERK signalling and epithelial-mesenchymal transition by Rac1b Sci Rep, 2017;7(1):17313. 2017 [PMID: 29229918] (Western Blot, Human)
• BA Rodrigues, VR Muñoz, GK Kuga, RC Gaspar, SCBR Nakandakar, BM Crisol, JD Botezelli, LSS Pauli, ASR da Silva, LP de Moura, DE Cintra, ER Ropelle, JR Pauli Obesity Increases Mitogen-Activated Protein Kinase Phosphatase-3 Levels in the Hypothalamus of Mice Front Cell Neurosci, 2017;11(0):313. 2017 [PMID: 29062272] (Western Blot, Mouse)
• Lo H, Lai T, Li C, Wu W TNF-alpha induces CXCL1 chemokine expression and release in human vascular endothelial cells in vitro via two distinct signaling pathways. Acta Pharmacol Sin, 2014;35(3):339-50. 2014 [PMID: 24487964] (Not Specified, Human)
• Lo HM, Tsai YJ, Du WY, Tsou CJ, Wu WB A naturally occurring carotenoid, lutein, reduces PDGF and H2O2 signaling and compromised migration in cultured vascular smooth muscle cells. J. Biomed. Sci., 2012;19(0):18. 2012 [PMID: 22313606] (Western Blot, Rat)
• Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer. Clin. Cancer Res., 2011;17(11):3619-30. 2011 [PMID: 21482691] (Western Blot, Human)
• Donev IS, Wang W, Yamada T, Li Q, Takeuchi S, Matsumoto K, Yamori T, Nishioka Y, Sone S, Yano S Transient PI3K Inhibition Induces Apoptosis and Overcomes HGF-Mediated Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer. Clin. Cancer Res., 2011;17(8):2260-9. 2011 [PMID: 21220474] (Western Blot, Human)
• Montecucco F, Lenglet S, Braunersreuther V, Burger F, Pelli G, Bertolotto M, Mach F, Steffens S CB(2) cannabinoid receptor activation is cardioprotective in a mouse model of ischemia/reperfusion. J. Mol. Cell. Cardiol., 2009;46(5):612-20. 2009 [PMID: 19162037] (Western Blot, Mouse)

Review for ERK1/ERK2 Antibody (AF1576) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous WB Mouse 01/05/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 24382894
• Species: Mouse

Blocking

• Blocking Details: See PMID 24382894

Details

• Detection Notes: See PMID 24382894

Comments

• Comments: Published: PMID 24382894