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ERK1/ERK2 [p Thr185, p Tyr187, p Thr202, p Tyr204] Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.1 µg/mL of Rabbit ...read more
Jurkat human acute T cell leukemia cell line were untreated (yellow line open histogram) or treated with 200 ng/mL PMA for 15 minutes (filled histogram) and stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 ...read more
ERK1/ERK2 was detected in perfusion fixed frozen sections of rat brain (cortex) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. A specific band was detected for ERK1 (T202/Y204)/ERK2 (T185/Y187) ...read more
Biochemical analysis of ErbB signaling.Immunoblot analysis performed on P90 kidneys from Cdh16Cre::Tfebfs mice (A) and P90 Cdh16CreErt2::Tfebfs animals induced with tamoxifen at P14 (B) and at P30 (C), respectively. ...read more
Activation of ErbB and WNT signaling pathways in kidneys from Cdh16Cre::Tfebfs mice.Transcriptional and biochemical analyses were performed on Cdh16Cre and Cdh16Cre::Tfebfs mice. (A,B) Tables show the relative increase ...read more
Biochemical analysis of ErbB signaling.Immunoblot analysis performed on P90 kidneys from Cdh16Cre::Tfebfs mice (A) and P90 Cdh16CreErt2::Tfebfs animals induced with tamoxifen at P14 (B) and at P30 (C), respectively. ...read more
Biochemical analysis of ErbB signaling.Immunoblot analysis performed on P90 kidneys from Cdh16Cre::Tfebfs mice (A) and P90 Cdh16CreErt2::Tfebfs animals induced with tamoxifen at P14 (B) and at P30 (C), respectively. ...read more
beta -blockers inhibit the proliferation of breast cancer cell lines(A) A dose curve of propranolol was administered to a panel of breast cancer cell lines and normal primary mammary epithelial cells (HMECS) and ...read more
Activation of ErbB and WNT signaling pathways in kidneys from Cdh16Cre::Tfebfs mice.Transcriptional and biochemical analyses were performed on Cdh16Cre and Cdh16Cre::Tfebfs mice. (A,B) Tables show the relative increase ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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ERK1/ERK2 [p Thr185, p Tyr187, p Thr202, p Tyr204] Antibody [Unconjugated] Summary

Description
Antibody detects ERK1 [p Thr202, p Tyr204] / ERK2 [p Thr185, p Tyr187]
Immunogen
Phosphopeptide containing ERK1 T202/Y204 sites
Modification
p Thr185, p Tyr187, p Thr202, p Tyr204
Specificity
Detects human, mouse and rat Phospho-ERK1/ERK2 when dually phosphorylated at T202/Y204 and T185/Y187, respectively.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 4
using
AF1018 in the following applications:

Publications
Read Publications using
AF1018 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ERK1/ERK2 [p Thr185, p Tyr187, p Thr202, p Tyr204] Antibody [Unconjugated]

  • ERK1/ERK2

Background

ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.
  1. Roskoski Jr., R. (2012) Pharmacol Res. 66:105.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERK1/ERK2 Antibody (AF1018)(41)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC, IHC, IHC-P, Simple Western, Western Blot.


Filter By Application
ICC
(1)
IHC
(2)
IHC-P
(1)
Simple Western
(3)
Western Blot
(19)
All Applications
Filter By Species
Human
(15)
Mouse
(7)
Rat
(4)
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Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using AF1018 Applications Species
Jinyu Zhang, Ye Lu, Min Jia, Yuying Bai, Lulu Sun, Ziqing Dong et al. Kappa opioid receptor in nucleus accumbens regulates depressive-like behaviors following prolonged morphine withdrawal in mice iScience 2023-09-15 [PMID: 37636073]
Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury iScience 2023-06-16 [PMID: 37332598]
Aurimas Stulpinas, Matas Sereika, Aida Vitkeviciene, Ausra Imbrasaite, Natalija Krestnikova, Audrone V. Kalvelyte Crosstalk between protein kinases AKT and ERK1/2 in human lung tumor-derived cell models Frontiers in Oncology 2023-01-04 [PMID: 36686779]
M Okada, K Fukuyama, E Motomura Dose-Dependent Biphasic Action of Quetiapine on AMPK Signalling via 5-HT7 Receptor: Exploring Pathophysiology of Clinical and Adverse Effects of Quetiapine International Journal of Molecular Sciences, 2022-08-14;23(16):. 2022-08-14 [PMID: 36012369] (Simple Western, Rat) Simple Western Rat
Rikako Ishigamori, Mie Naruse, Akihiro Hirata, Yoshiaki Maru, Yoshitaka Hippo, Toshio Imai The potential of organoids in toxicologic pathology: Histopathological and immunohistochemical evaluation of a mouse normal tissue-derived organoid-based carcinogenesis model Journal of Toxicologic Pathology 2022-04-22 [PMID: 35832903]
K Fukuyama, E Motomura, M Okada Brexpiprazole Reduces 5-HT7 Receptor Function on Astroglial Transmission Systems International Journal of Molecular Sciences, 2022-06-12;23(12):. 2022-06-12 [PMID: 35743014] (Simple Western, Rat) Simple Western Rat
Hung-Pin Chang, Jen-Tang Sun, Chiao-Yin Cheng, Yao-Jen Liang, Yen-Lin Chen High Mobility Group A 1 Expression as a Poor Prognostic Marker Associated with Tumor Invasiveness in Gastric Cancer Life (Basel) 2022-05-09 [PMID: 35629376]
T Shiroyama, K Fukuyama, M Okada Distinct Effects of Escitalopram and Vortioxetine on Astroglial L-Glutamate Release Associated with Connexin43 International Journal of Molecular Sciences, 2021-09-16;22(18):. 2021-09-16 [PMID: 34576176] (Simple Western, Rat) Simple Western Rat
Y Machida, T Imai Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c Trp53 knockout mice Oncology Letters, 2021-08-16;22(4):738. 2021-08-16 [PMID: 34466150] (IHC, Mouse) IHC Mouse
Massman LJ, Pereckas M, Zwagerman NT, Olivier-Van Stichelen S. O-GlcNAcylation Is Essential for Rapid Pomc Expression and Cell Proliferation in Corticotropic Tumor Cells Endocrinology 2021-12-01 [PMID: 34418053]
Show All 41 Publications.

Review for ERK1/ERK2 Antibody (AF1018) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using AF1018:
Filter by Applications
WB
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Rat 01/05/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23559668
SpeciesRat

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Verified Customer
01/05/2015
Application: WB
Species: Rat