ERK1/ERK2 [p Thr185, p Tyr187, p Thr202, p Tyr204] Antibody [Unconjugated]

Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) was detected in immersion fixed paraffin-embedded sections of human Cortex using Rabbit Anti-Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) , Polyclonal Antibody (Catalog #AF1018) at 25ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus of the neuron. Protocol available in COMET™ Panel Builder.

Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) was detected in immersion fixed paraffin-embedded sections of mouse Cerebellum using Rabbit Anti-Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187), Polyclonal Antibody (Catalog #AF1018) at 25ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm of the neuronal processes. Protocol available in COMET™ Panel Builder.​​

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for for the indicated times. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204) and ERK2 (T185/Y187) at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Jurkat human acute T cell leukemia cell line were untreated (yellow line open histogram) or treated with 200 ng/mL PMA for 15 minutes (filled histogram) and stained with Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018) or control antibody (Catalog # AB-105-C, blue line open histogram), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with PFA and permeabilized with ice-cold methanol.

ERK1/ERK2 was detected in perfusion fixed frozen sections of rat brain (cortex) using 15 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018) overnight at 4 °C. Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. A specific band was detected for ERK1 (T202/Y204)/ERK2 (T185/Y187) at approximately 44 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Biochemical analysis of ErbB signaling.Immunoblot analysis performed on P90 kidneys from Cdh16Cre::Tfebfs mice (A) and P90 Cdh16CreErt2::Tfebfs animals induced with tamoxifen at P14 (B) and at P30 (C), respectively. Each replicate is a different biological sample. ErbB was analyzed by quantifying phosphoAKT (Ser473) to total AKT, and phosphoERK1 (T202/Y204)/ERK2(T185/Y187) to total ERK; graphs are the densitometry quantifications of Western blot bands normalized to wild-type line and are shown as an average (± SEM) (*p<0.05, **p<0.01, ***p<0.001, two-sided Student’s t test).DOI://dx.doi.org/10.7554/eLife.17047.011 Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/17047), licensed under a CC-BY license. Not internally tested by R&D Systems.

Activation of ErbB and WNT signaling pathways in kidneys from Cdh16Cre::Tfebfs mice.Transcriptional and biochemical analyses were performed on Cdh16Cre and Cdh16Cre::Tfebfs mice. (A,B) Tables show the relative increase of genes related to the ErbB (A) and WNT (B) pathways in the microarray analyses performed on kidneys from P0 Cdh16Cre::Tfebfs mice. Graphs show real-time PCR validations performed on kidneys from Cdh16Cre::Tfebfs mice at different stages (P0, P12, P30). Data are shown as the average (± SEM) of at least three Cdh16Cre::Tfebfs mice normalized versus wild-type mice. (C,D) Immunoblot analyses performed on (C) P30 kidney tissues and (D) primary kidney cells isolated from Cdh16Cre::Tfebfs mice to evaluate ErbB and WNT activation status. Each replicate is a distinct biological sample. ErbB signaling was assessed by looking at phosphoAKT (Ser473) to total AKT ratio, and phosphoERK1 (T202/Y204)/ERK2(T185/Y187) to total ERK ratio; WNT signaling was assessed by quantifying beta -catenin and CCND1 (Cyclin D1) protein levels. Graphs represent the densitometry quantification of Western blot bands. Values are normalized to actin when not specified and are shown as an average (± SEM) (*p<0.05, **p<0.01, ***p<0.001, two-sided, Student’s t test).DOI://dx.doi.org/10.7554/eLife.17047.00710.7554/eLife.17047.008Figure 3—source data 1.Complete list of 294 genes (represented by 361 probesets) significantly induced (FDR≤0.05) in the KSP_P0 microarray dataset (GSE62977).The genes are ranked by decreasing signed ratio (KSP_P0/CTL).DOI://dx.doi.org/10.7554/eLife.17047.00810.7554/eLife.17047.009Figure 3—source data 2.Complete list of 628 genes (represented by 729 probesets) significantly induced (FDR≤0.05) in the KSP_P14 microarray dataset (GSE63376).The genes are ranked by decreasing signed ratio (KSP_P14/CTL).DOI://dx.doi.org/10.7554/eLife.17047.009Complete list of 294 genes (represented by 361 probesets) significantly induced (FDR≤0.05) in the KSP_P0 microarray dataset (GSE62977).The genes are ranked by decreasing signed ratio (KSP_P0/CTL).DOI://dx.doi.org/10.7554/eLife.17047.008Complete list of 628 genes (represented by 729 probesets) significantly induced (FDR≤0.05) in the KSP_P14 microarray dataset (GSE63376).The genes are ranked by decreasing signed ratio (KSP_P14/CTL).DOI://dx.doi.org/10.7554/eLife.17047.009ErbB and WNT transcriptional profiles in Cdh16CreErt2::Tfebfs mice.Transcriptional analyses performed on Cdh16CreErt2::Tfebfs mice. (A,B) mRNA levels of previously validated genes belonging to the WNT (left graphs) and ErbB (right graphs) signaling pathways assessed in P90 Cdh16CreErt2::Tfebfs mice induced at (A) P14 and at (B) P30 with tamoxifen respectively. Data are shown as the average (± SEM) of at least Cdh16CreErt2::Tfebfs mice and values are normalized to the wild-type line. (*p<0.05, **p<0.01, ***p<0.001, two-sided Student’s t test).DOI://dx.doi.org/10.7554/eLife.17047.010Biochemical analysis of ErbB signaling.Immunoblot analysis performed on P90 kidneys from Cdh16Cre::Tfebfs mice (A) and P90 Cdh16CreErt2::Tfebfs animals induced with tamoxifen at P14 (B) and at P30 (C), respectively. Each replicate is a different biological sample. ErbB was analyzed by quantifying phosphoAKT (Ser473) to total AKT, and phosphoERK1 (T202/Y204)/ERK2(T185/Y187) to total ERK; graphs are the densitometry quantifications of Western blot bands normalized to wild-type line and are shown as an average (± SEM) (*p<0.05, **p<0.01, ***p<0.001, two-sided Student’s t test).DOI://dx.doi.org/10.7554/eLife.17047.011 Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/17047), licensed under a CC-BY license. Not internally tested by R&D Systems.

Biochemical analysis of ErbB signaling.Immunoblot analysis performed on P90 kidneys from Cdh16Cre::Tfebfs mice (A) and P90 Cdh16CreErt2::Tfebfs animals induced with tamoxifen at P14 (B) and at P30 (C), respectively. Each replicate is a different biological sample. ErbB was analyzed by quantifying phosphoAKT (Ser473) to total AKT, and phosphoERK1 (T202/Y204)/ERK2(T185/Y187) to total ERK; graphs are the densitometry quantifications of Western blot bands normalized to wild-type line and are shown as an average (± SEM) (*p<0.05, **p<0.01, ***p<0.001, two-sided Student’s t test).DOI://dx.doi.org/10.7554/eLife.17047.011 Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/17047), licensed under a CC-BY license. Not internally tested by R&D Systems.

Biochemical analysis of ErbB signaling.Immunoblot analysis performed on P90 kidneys from Cdh16Cre::Tfebfs mice (A) and P90 Cdh16CreErt2::Tfebfs animals induced with tamoxifen at P14 (B) and at P30 (C), respectively. Each replicate is a different biological sample. ErbB was analyzed by quantifying phosphoAKT (Ser473) to total AKT, and phosphoERK1 (T202/Y204)/ERK2(T185/Y187) to total ERK; graphs are the densitometry quantifications of Western blot bands normalized to wild-type line and are shown as an average (± SEM) (*p<0.05, **p<0.01, ***p<0.001, two-sided Student’s t test).DOI://dx.doi.org/10.7554/eLife.17047.011 Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/17047), licensed under a CC-BY license. Not internally tested by R&D Systems.

beta -blockers inhibit the proliferation of breast cancer cell lines(A) A dose curve of propranolol was administered to a panel of breast cancer cell lines and normal primary mammary epithelial cells (HMECS) and viability was assessed. (B) Immunofluorescent detection of Ki-67 protein expression in control or propranolol-treated (18 μM) SK-BR-3 breast cancer cells after 24 hours. Hoechst 33342 was used as a nuclear counterstain. (C) Heatmap depicting phospho-MAPK antibody array results testing the status of 24 kinases in SK-BR-3 cells subjected to 24 hours control or propranolol (18 μM). (red = upregulated; green = downregulated; black = no detectable expression). (D) Western blot confirmation of the antibody array results in SK-BR-3 cells subjected to 24 hours control or propranolol (18 μM). (D) Western blot analysis of cell lysates from SK-BR-3 cells subjected to 24 hours control or propranolol (18 μM), confirming the phosphorylation events identified in the antibody array. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.14119), licensed under a CC-BY license. Not internally tested by R&D Systems.

Activation of ErbB and WNT signaling pathways in kidneys from Cdh16Cre::Tfebfs mice.Transcriptional and biochemical analyses were performed on Cdh16Cre and Cdh16Cre::Tfebfs mice. (A,B) Tables show the relative increase of genes related to the ErbB (A) and WNT (B) pathways in the microarray analyses performed on kidneys from P0 Cdh16Cre::Tfebfs mice. Graphs show real-time PCR validations performed on kidneys from Cdh16Cre::Tfebfs mice at different stages (P0, P12, P30). Data are shown as the average (± SEM) of at least three Cdh16Cre::Tfebfs mice normalized versus wild-type mice. (C,D) Immunoblot analyses performed on (C) P30 kidney tissues and (D) primary kidney cells isolated from Cdh16Cre::Tfebfs mice to evaluate ErbB and WNT activation status. Each replicate is a distinct biological sample. ErbB signaling was assessed by looking at phosphoAKT (Ser473) to total AKT ratio, and phosphoERK1 (T202/Y204)/ERK2(T185/Y187) to total ERK ratio; WNT signaling was assessed by quantifying beta -catenin and CCND1 (Cyclin D1) protein levels. Graphs represent the densitometry quantification of Western blot bands. Values are normalized to actin when not specified and are shown as an average (± SEM) (*p<0.05, **p<0.01, ***p<0.001, two-sided, Student’s t test).DOI://dx.doi.org/10.7554/eLife.17047.00710.7554/eLife.17047.008Figure 3—source data 1.Complete list of 294 genes (represented by 361 probesets) significantly induced (FDR≤0.05) in the KSP_P0 microarray dataset (GSE62977).The genes are ranked by decreasing signed ratio (KSP_P0/CTL).DOI://dx.doi.org/10.7554/eLife.17047.00810.7554/eLife.17047.009Figure 3—source data 2.Complete list of 628 genes (represented by 729 probesets) significantly induced (FDR≤0.05) in the KSP_P14 microarray dataset (GSE63376).The genes are ranked by decreasing signed ratio (KSP_P14/CTL).DOI://dx.doi.org/10.7554/eLife.17047.009Complete list of 294 genes (represented by 361 probesets) significantly induced (FDR≤0.05) in the KSP_P0 microarray dataset (GSE62977).The genes are ranked by decreasing signed ratio (KSP_P0/CTL).DOI://dx.doi.org/10.7554/eLife.17047.008Complete list of 628 genes (represented by 729 probesets) significantly induced (FDR≤0.05) in the KSP_P14 microarray dataset (GSE63376).The genes are ranked by decreasing signed ratio (KSP_P14/CTL).DOI://dx.doi.org/10.7554/eLife.17047.009ErbB and WNT transcriptional profiles in Cdh16CreErt2::Tfebfs mice.Transcriptional analyses performed on Cdh16CreErt2::Tfebfs mice. (A,B) mRNA levels of previously validated genes belonging to the WNT (left graphs) and ErbB (right graphs) signaling pathways assessed in P90 Cdh16CreErt2::Tfebfs mice induced at (A) P14 and at (B) P30 with tamoxifen respectively. Data are shown as the average (± SEM) of at least Cdh16CreErt2::Tfebfs mice and values are normalized to the wild-type line. (*p<0.05, **p<0.01, ***p<0.001, two-sided Student’s t test).DOI://dx.doi.org/10.7554/eLife.17047.010Biochemical analysis of ErbB signaling.Immunoblot analysis performed on P90 kidneys from Cdh16Cre::Tfebfs mice (A) and P90 Cdh16CreErt2::Tfebfs animals induced with tamoxifen at P14 (B) and at P30 (C), respectively. Each replicate is a different biological sample. ErbB was analyzed by quantifying phosphoAKT (Ser473) to total AKT, and phosphoERK1 (T202/Y204)/ERK2(T185/Y187) to total ERK; graphs are the densitometry quantifications of Western blot bands normalized to wild-type line and are shown as an average (± SEM) (*p<0.05, **p<0.01, ***p<0.001, two-sided Student’s t test).DOI://dx.doi.org/10.7554/eLife.17047.011 Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/17047), licensed under a CC-BY license. Not internally tested by R&D Systems.

Biochemical analysis of ErbB signaling.Immunoblot analysis performed on P90 kidneys from Cdh16Cre::Tfebfs mice (A) and P90 Cdh16CreErt2::Tfebfs animals induced with tamoxifen at P14 (B) and at P30 (C), respectively. Each replicate is a different biological sample. ErbB was analyzed by quantifying phosphoAKT (Ser473) to total AKT, and phosphoERK1 (T202/Y204)/ERK2(T185/Y187) to total ERK; graphs are the densitometry quantifications of Western blot bands normalized to wild-type line and are shown as an average (± SEM) (*p<0.05, **p<0.01, ***p<0.001, two-sided Student’s t test).DOI://dx.doi.org/10.7554/eLife.17047.011 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27668431), licensed under a CC-BY license. Not internally tested by R&D Systems.

Activation of ErbB and WNT signaling pathways in kidneys from Cdh16Cre::Tfebfs mice.Transcriptional and biochemical analyses were performed on Cdh16Cre and Cdh16Cre::Tfebfs mice. (C,D) Immunoblot analyses performed on (C) P30 kidney tissues and (D) primary kidney cells isolated from Cdh16Cre::Tfebfs mice to evaluate ErbB and WNT activation status. Each replicate is a distinct biological sample. ErbB signaling was assessed by looking at phosphoAKT (Ser473) to total AKT ratio, and phosphoERK1 (T202/Y204)/ERK2(T185/Y187) to total ERK ratio; WNT signaling was assessed by quantifying beta -catenin and CCND1 (Cyclin D1) protein levels. Graphs represent the densitometry quantification of Western blot bands. Values are normalized to actin when not specified and are shown as an average (± SEM) (*p<0.05, **p<0.01, ***p<0.001, two-sided, Student’s t test). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27668431), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, mIF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

ERK1/ERK2 [p Thr185, p Tyr187, p Thr202, p Tyr204] Antibody [Unconjugated] Summary

• Description: Antibody detects ERK1 [p Thr202, p Tyr204] / ERK2 [p Thr185, p Tyr187]
• Immunogen: Phosphopeptide containing ERK1 T202/Y204 sites
• Modification: p Thr185, p Tyr187, p Thr202, p Tyr204
• Specificity: Detects human, mouse and rat Phospho-ERK1/ERK2 when dually phosphorylated at T202/Y204 and T185/Y187, respectively.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Multiplex Immunofluorescence 25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ERK1/ERK2 [p Thr185, p Tyr187, p Thr202, p Tyr204] Antibody [Unconjugated]

• ERK1
• ERK-1
• ERK1/ERK2
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAPK 1
• Microtubule-associated protein 2 kinase
• mitogen-activated protein kinase 3
• p44erk1
• p44-ERK1
• p44mapk
• p44-MAPK
• PRKM3

Background

ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.

1. Roskoski Jr., R. (2012) Pharmacol Res. 66:105.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERK1/ERK2 Antibody (AF1018)(43)

Publications using AF1018

• Ang, SY;Shih, CY;Ho, H;Chen, YL;Sun, JT;Cheng, CY; EphA5 Expression Predicts Better Survival Despite an Association with Proliferative Activity in Endometrial Cancer Journal of clinical medicine 2025-07-29 [PMID: 40806980] (Immunohistochemistry, Human)
• Paramasivam, P;Choi, SW;Poddar, R;Paul, S; Impairment of neuronal tyrosine phosphatase STEP worsens post-ischemic inflammation and brain injury under hypertensive condition Journal of neuroinflammation 2024-10-22 [PMID: 39438980] (Immunohistochemistry, Western Blot, Rat)
• Fukuyama, K;Motomura, E;Okada, M; Age-Dependent Activation of Purinergic Transmission Contributes to the Development of Epileptogenesis in ADSHE Model Rats Biomolecules 2024-02-08 [PMID: 38397441] (Simple Western, Rat, Transgenic Rat)
• Jinyu Zhang, Ye Lu, Min Jia, Yuying Bai, Lulu Sun, Ziqing Dong et al. Kappa opioid receptor in nucleus accumbens regulates depressive-like behaviors following prolonged morphine withdrawal in mice iScience 2023-09-15 [PMID: 37636073]
• Qian Xia, Meng Mao, Gaofeng Zhan, Zhenzhao Luo, Yin Zhao, Xing Li SENP3-mediated deSUMOylation of c-Jun facilitates microglia-induced neuroinflammation after cerebral ischemia and reperfusion injury iScience 2023-06-16 [PMID: 37332598] (Western Blot, Mouse)
• Aurimas Stulpinas, Matas Sereika, Aida Vitkeviciene, Ausra Imbrasaite, Natalija Krestnikova, Audrone V. Kalvelyte Crosstalk between protein kinases AKT and ERK1/2 in human lung tumor-derived cell models Frontiers in Oncology 2023-01-04 [PMID: 36686779] (Western Blot, Human)
• M Okada, K Fukuyama, E Motomura Dose-Dependent Biphasic Action of Quetiapine on AMPK Signalling via 5-HT7 Receptor: Exploring Pathophysiology of Clinical and Adverse Effects of Quetiapine International Journal of Molecular Sciences, 2022-08-14;23(16):. 2022-08-14 [PMID: 36012369] (Simple Western, Rat)
• Rikako Ishigamori, Mie Naruse, Akihiro Hirata, Yoshiaki Maru, Yoshitaka Hippo, Toshio Imai The potential of organoids in toxicologic pathology: Histopathological and immunohistochemical evaluation of a mouse normal tissue-derived organoid-based carcinogenesis model Journal of Toxicologic Pathology 2022-04-22 [PMID: 35832903]
• K Fukuyama, E Motomura, M Okada Brexpiprazole Reduces 5-HT7 Receptor Function on Astroglial Transmission Systems International Journal of Molecular Sciences, 2022-06-12;23(12):. 2022-06-12 [PMID: 35743014] (Simple Western, Rat)
• Hung-Pin Chang, Jen-Tang Sun, Chiao-Yin Cheng, Yao-Jen Liang, Yen-Lin Chen High Mobility Group A 1 Expression as a Poor Prognostic Marker Associated with Tumor Invasiveness in Gastric Cancer Life (Basel) 2022-05-09 [PMID: 35629376]
• T Shiroyama, K Fukuyama, M Okada Distinct Effects of Escitalopram and Vortioxetine on Astroglial L-Glutamate Release Associated with Connexin43 International Journal of Molecular Sciences, 2021-09-16;22(18):. 2021-09-16 [PMID: 34576176] (Simple Western, Rat)
• Y Machida, T Imai Different properties of mammary carcinogenesis induced by two chemical carcinogens, DMBA and PhIP, in heterozygous BALB/c Trp53 knockout mice Oncology Letters, 2021-08-16;22(4):738. 2021-08-16 [PMID: 34466150] (IHC, Mouse)
• Massman LJ, Pereckas M, Zwagerman NT, Olivier-Van Stichelen S. O-GlcNAcylation Is Essential for Rapid Pomc Expression and Cell Proliferation in Corticotropic Tumor Cells Endocrinology 2021-12-01 [PMID: 34418053]
• E Soleimanpo, JR Bergado Ac, P Landgraf, D Mayer, E Dankert, DC Dieterich, M Fendt Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning Cells, 2021-01-26;10(2):. 2021-01-26 [PMID: 33530478] (Western Blot, Rat)
• LZ Rao, Y Wang, L Zhang, G Wu, L Zhang, FX Wang, LM Chen, F Sun, S Jia, S Zhang, Q Yu, JH Wei, HR Lei, T Yuan, J Li, X Huang, B Cheng, J Zhao, Y Xu, BW Mo, CY Wang, H Zhang IL-24 deficiency protects mice against bleomycin-induced pulmonary fibrosis by repressing IL-4-induced M2 program in macrophages Cell Death Differ, 2020-11-03;0(0):. 2020-11-03 [PMID: 33144678] (IHC, Mouse)
• Fukuyama K, Okada M. Age-Dependent and Sleep/Seizure-Induced Pathomechanisms of Autosomal Dominant Sleep-Related Hypermotor Epilepsy International Journal of Molecular Sciences 2020-10-30 [PMID: 33143372]
• Junling Liu, Jianyun Liu, Mingming Bai, Hui Wang Protective effect of puerarin against burn‑induced heart injury in rats Experimental and Therapeutic Medicine 2020-04-29 [PMID: 32536996]
• Ana I. Sánchez, Mary A. García-Acero, Angela Paredes, Rossi Quero, Rita I. Ortega, Jorge A. Rojas et al. Immunodeficiency in a Patient with 22q11.2 Distal Deletion Syndrome and a p.Ala7dup Variant in the <b><i>MAPK1</i></b> Gene Molecular Syndromology 2020-01-01 [PMID: 32256297]
• C Niersmann, K Röhrig, M Blüher, M Roden, C Herder, M Carstensen Increased Release of Proinflammatory Proteins in Primary Human Adipocytes and Activation of the Inflammatory NF?B, p38, and ERK Pathways upon Omentin Treatment Obes Facts, 2020-04-06;13(2):221-236. 2020-04-06 [PMID: 32252061] (Western Blot, Human)
• JF Burgueño, JK Lang, AM Santander, I Fernández, E Fernández, J Zaias, MT Abreu Fluid supplementation accelerates epithelial repair during chemical colitis PLoS ONE, 2019-04-19;14(4):e0215387. 2019-04-19 [PMID: 31002683] (IHC-P, Mouse)
• Gennaro Napolitano, Alessandra Esposito, Heejun Choi, Maria Matarese, Valerio Benedetti, Chiara Di Malta et al. mTOR-dependent phosphorylation controls TFEB nuclear export Nature Communications 2018-08-17 [PMID: 30120233]
• SM Herbrich, S Kannan, RM Nolo, M Hornbaker, J Chandra, PA Zweidler-M Characterization of TRKA signaling in acute myeloid leukemia Oncotarget, 2018-07-10;9(53):30092-30105. 2018-07-10 [PMID: 30046390] (Western Blot, Human)
• Z Wan, C Xu, X Chen, H Xie, Z Li, J Wang, X Ji, H Chen, Q Ji, S Shaheen, Y Xu, F Wang, Z Tang, JS Zheng, W Chen, J Lou, W Liu PI(4,5)P2 determines the threshold of mechanical force-induced B cell activation J. Cell Biol., 2018-04-23;0(0):. 2018-04-23 [PMID: 29685902] (ICC, Mouse)
• NM Scheers, DIA Pereira, N Faria, JJ Powell Ferric citrate and ferric EDTA but not ferrous sulfate drive amphiregulin-mediated activation of the MAP kinase ERK in gut epithelial cancer cells Oncotarget, 2018-03-30;9(24):17066-17077. 2018-03-30 [PMID: 29682205] (Western Blot, Human)
• KM Wilson, AM Jagger, M Walker, E Seinkmane, JM Fox, R Kröger, P Genever, D Ungar Glycans modify mesenchymal stem cell differentiation to impact the function of resulting osteoblasts J. Cell. Sci., 2018-02-14;0(0):. 2018-02-14 [PMID: 29361539] (Western Blot, Human)
• Sachiko Arai, Kenji Kita, Azusa Tanimoto, Shinji Takeuchi, Koji Fukuda, Hiroshi Sato et al. In vitro and in vivo anti-tumor activity of alectinib in tumor cells with NCOA4-RET Oncotarget 2017-09-26 [PMID: 29088743]
• BA Rodrigues, VR Muñoz, GK Kuga, RC Gaspar, SCBR Nakandakar, BM Crisol, JD Botezelli, LSS Pauli, ASR da Silva, LP de Moura, DE Cintra, ER Ropelle, JR Pauli Obesity Increases Mitogen-Activated Protein Kinase Phosphatase-3 Levels in the Hypothalamus of Mice Front Cell Neurosci, 2017-10-09;11(0):313. 2017-10-09 [PMID: 29062272] (Western Blot, Mouse)
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Review for ERK1/ERK2 Antibody (AF1018) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 01/05/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23559668
• Species: Rat