ERK1/2 Antibody

Western Blot: ERK1/2 Antibody [NBP2-12728] - analysis of Human Cell line lysates showing detection of ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NBP2-12728] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.

Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP2-12728] - Tested on Bouin's fixed paraffin embedded backskin sections of transgenic mice.

Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NBP2-12728] - Tissue: backskin. Species: Mouse. ERK1 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm. This image is courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.

Immunocytochemistry/Immunofluorescence: ERK1/2 Antibody [NBP2-12728] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Erk1/2 Polyclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 120 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Erk1/2 Antibody. (C) Composite.

Immunohistochemistry-Paraffin: ERK1/2 Antibody [NBP2-12728] - Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody at 1:25000 for 12 hours at 4-C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200 uL for 2 min at RT

Product Discontinued

ERK1/2 Antibody Summary

• Immunogen: A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
• Localization: Cytoplasm , Nucleus
• Specificity: Detects approx 44kda (ERK1) and approx 42kDa (ERK2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:5000
• Application Notes: A 1:1000 dilution of Erk1/2 Antibody was sufficient for detection of ERK1/2 in 20 ug of HeLa cell lysate by ECL immunoblot analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ERK1/2 Antibody

• EC 2.7.11
• ERK-1
• ERK1p44-MAPK
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAP kinase 1
• MAP kinase 3
• MAP kinase isoform p44
• MAPK 1
• MAPK 3
• MGC20180
• Microtubule-associated protein 2 kinase
• Mitogen-activated protein kinase 1
• mitogen-activated protein kinase 3
• p44erk1
• p44-ERK1
• p44mapk
• PRKM3EC 2.7.11.24

Background

Certificate of Analysis, 1: 5000 dilution was sufficient for detection of MAPKAP Kinase 3 in 20ug of HeLa cell lysate by ECL immunoblot analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERK1/2 Antibody (NBP2-12728)(1)

Publication using NBP2-12728

• Batalha IM, Maylem ERS, Spicer LJ et al. Effects of asprosin on estradiol and progesterone secretion and proliferation of bovine granulosa cells Molecular and cellular endocrinology 2023-04-05 [PMID: 36822263] (Simple Western, Bovine)

Review for ERK1/2 Antibody (NBP2-12728) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Bovine.

reviewed by: Verified Customer Simple Western Bovine 12/15/2022

Summary

• Application: Simple Western
• Sample Tested: Bovine granulosa cell
• Species: Bovine
• Lot: PE185042

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: MAPK3