ERF Antibody - BSA Free

Immunohistochemistry-Paraffin: ERF Antibody [NB100-88120] - FFPE section of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-ERF (NB100-88120). Detection: DAB

Immunoprecipitation: ERF Antibody [NB100-88120] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-88120 used for IP at 3 mcg/mg lysate. ERF was also immunoprecipitated by NB100-88119, which recognizes an upstream epitope.

• Reactivity: Hu, Mu
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ERF Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 498 and 548 of human Ets2 repressor factor using the numbering given in entry NP_006485.2 (GeneID 2077).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ERF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2 - 5 ug/mg lysate
• Application Notes: Immunohistochemistry-Paraffin: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  
  Western Blot: This antibody has not performed satisfactorily when used for western blot of ERF in crude preparations (e.g. whole cell lysate). This antibody can be used for western blot of enriched (e.g. immunoprecipitated) sources of ERF.
• Control:

  ERF Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ERF Antibody - BSA Free

• Ets2 repressor factorPE-2PE2ETS domain-containing transcription factor ERF

Background

Members of the ETS family of transcription factors, such as ERF, regulate cell proliferation and differentiation. They share a highly conserved DNA-binding domain, the ETS domain, that recognizes the sequence GGAA/T (de Castro et al., 1997 [PubMed 9192842]). For further information on ETS transcription factors, see ETS1 (MIM 164720).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ERF
• Entrez:
  • 2077(Human)
• Uniprot:
  • NP_006485.2(Human)